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1、Biochimie,69(1987)591-600591©Soci~t~deChimiebiologique/Elsevier,ParisEntericcoronavirusTGEV:partialsequenceofthegenomicRNA,itsorganizationandexpressionDenisRASSCHAERT,JacquelineGELFIandHubertLAUDE*InstitutNationaldelaRechercheAgronomique,StationdeRecherchesdeVirologieetd'Immunologie,F-78850Thiverv
2、ai-Grignon,France(Received27-3-198%acceptedafterrevision24-4-1987)Summary-Thesequenceofthe3'-most8300nucleotidesofthegenomeRNAofthePurdue-115strainofthetransmissiblegastroenteritisvirusTGEV,aporcinecoronavirus,wasdeterminedfromcDNAclones.Theavailablesequencecorrespondstothepartofthegenome(totallen
3、gth>20kb)expressedthroughsubgenomicmRNAs.The5subgenomicandthegenomicRNAspeciesdetectedinTGEV-infectedcellsforma3'-coterminal'nested'structure,auniquefeatureofCoronaviridae.ThetranscriptioninitiationsiteoftheTGEVsubgenomicRNAsappearstoinvolvethehexamericse-quence5'CTAAAC,whichispresentupstreamfrome
4、achcodingregion.Inadditiontothepreviouslyiden-tifiedgenesencodingthethreestructuralproteins,E2,E1andN,tworegions,X1andX2,correspondingtothenon-overlappingportionofmRNAs4and3,maycodeforsofarunidentifiednon-structuralpolypep-tides.ThepredictedX1polypeptide(9.2kDa)ishighlyhydrophobic.Thesequenceofthe
5、X2regionallowsthetranslationoftwonon-overlappingproducts,i.e.,X2a(7.7kDa)andX2b(18.8kDa).NoRNAspeciesliabletoexpresstheextreme3'openreadingframeX3wasfound.coronavirusItransmissiblegastroenteritisITGEVImessengerRNAsIgenomestructure/gene._¢~_u_ence/n_on~-sWj___etu_ra__!polypeptides-1987)R~sumE-Virus
6、delagastro-entEritetransmissible(TGEV):sequencepartielle,organisationetexpressiondeI'ARNgEnomique.Las6quencedes8300nucl6otidesenr6gion3"deI'ARNgdnomiqueducoronavirusporcinTGEV(souchePurdue-llS)a6t66tablie~partirdeclonesd'ADNc.Parrapportaug6nomeentier(>20kb),celarecouvrel'ensembledessdquencesexprim
7、desparI'intermddiaired'ARNsmessagersdetaillesubgdnomique.Les5esp~cesd'ARNsubgdnomiquesetI'ARNgdnomiqued~tectdsdanslescellulesinfectdesformentdessdquencesembo~tdesco-terminalesen3",cequiestcaractdristiquedumodeder
