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1、57J.gen.Virol.(1987),68,5777.PrintedinGreatBritainKeywords:IBV/coronavirus/nueleotidesequenceCompletionoftheSequenceoftheGenomeoftheCoronavirusAvianInfectionsBronchitisVirusByM.E.G.BOURSNELL,*T.D.K.BROWN,I.J.FOULDS,P.F.GREEN,F.M.TOMLEYANDM.M.BINNSHoughtonPoultryResearchStation,Houghton,
2、Huntingdon,CambridgeshirePE172DA,U.K.(Accepted19September1986)SUMMARYThenucleotidesequencedeterminationofthegenomeoftheBeaudettestrainofthecoronavirusavianinfectiousbronchitisvirus(IBV)hasbeencompleted.Thecompletesequencehasbeenobtainedfrom17overlappingcDNAclones,the5'-mostofwhichcontai
3、nstheleadersequence(asdeterminedbydirectsequencingofthegenome)andthe3'-mostofwhichcontainsthepoly(A)tail.Approximately8kilobasesatthe3'endofthissequencehavealreadybeenpublished.ThesecontainthesequencesofmRNAsAtoEwithinwhicharethegenesforthespike,themembraneandthenucleocapsidpolypeptides
4、:themainstructuralcomponentsofthevirion.Theremainderofthesequence,equivalenttothe'unique'regionofmRNAF,issome20kilobasesinlengthandisthoughttocodeforapolymeraseorpolymeraseswhichareinvolvedinthereplicationofthegenomeandtheproductionofthesubgenomicmessengerRNAs.Thissequencecontainstwolar
5、geopenreadingframes,potentiallycodingforpolypeptidesofmolecularweights441000and300000.Unlikeotherlargeopenreadingframesinthevirus,the300000openreadingframeappearstohavenosubgenomicRNAassociatedwithitwhichwouldallowittobeatthe5'endofanmRNAspecies.Becauseofthis,andbecauseofthecharacterist
6、icsofthesequenceintheregionimmediatelyupstreamofitsstartcodon,othermechanismsoftranslation,suchasribosomeslippage,mustbepostulated.INTRODUCTIONAvianinfectiousbronchitisvirus(IBV)isthetypespeciesofthefamilyCoronaviridae(Siddelletal.,1983a).Coronavirusesareenveloped,pleomorphicparticleswi
7、thadistinctive'corona'ofclub-shapedsurfaceprojections,andalargesingle-strandedRNAgenomeofpositivepolarity(Siddelletal.,1983b).Ininfectedcells,inadditiontogenome-sizedRNA,anumberofsubgenomicRNAscanbedetectedwhichhaveacommon3'terminus,butextendfordifferentlengthsinthe5'direction,
