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1、VIROLOGY189,792-794(1992)NeuraminidaseTreatmentofAvianInfectiousBronchitisCoronavirusRevealsaHemagglutinatingActivityThatisDependentonSialicAcid-ContainingReceptorsonErythrocytesBEATESCHULTZE,*.’DAVIDCAVANAGH,tANDGEORGHERRLER**Institutfiirvirologie,Phi/tops-Universit;
2、itMarburg,Robert-Koch-Str.17,3550#arburg,Germany;andiAgricultura/andFoodResearchCouncil,InstituteforAnimalHealth,HoughtonLaboratory,Houghton,HuntingdonPE172DA,UKReceivedFebruary14,1992;acceptedApril16,1992Theinteractionofinfectiousbronchitisvirus(IBV)witherythrocytesw
3、asanalyzed.ThebindingactivityofIBVwasnotsufficienttoagglutinatechickenerythrocytes.However,itacquiredhemagglutinatingactivityaftertreatmentwithneuraminidasetoremove&,3-linkedN-acetylneuraminicacidfromthesurfaceofthevirion.Pretreatmentoferythro-cyteswithneuraminidasere
4、nderedthecellsresistanttoagglutinationbyIBV.Susceptibilitytoagglutinationwasrestoredbyresialylationofasialo-erythrocytestocontaina2,3-linkedsialicacid.TheseresultsindicatethatIBVat-tachestoreceptorsonetythrocytes,thecrucialdeterminantofwhichissialicacidol2,3-linkedtog
5、alactose.Incontrasttootherenvelopedviruseswithsuchabindingspecificity(influenzavirusesandparamyxoviruses)IBVlacksareceptor-destroyingenzyme.01992AcademicPress,Inc.TherearegreatvariationsamongmembersofthewithcommerciallyavailablephospholipaseC,weana-familyCoronaviridae
6、intheirabilitytoagglutinateredlyzedwhetherneuraminidasewaseffectiveinthisre-bloodceils.Somememberssuchasbovinecorona-spect.AsshowninTable1,whileuntreatedviruswasvirus(BCV),humancoronavirus(HCV-OC43),andhem-unabletoagglutinatechickenerythrocytes,highhem-agglutinatingen
7、cephalomyelitisvirus(HEV)arequiteagglutinationtiterswereobtainedaftertreatmentofviri-potenthemagglutinatingagents.StudieswithBCVonswithneuraminidasefromVibriocholerae.ThehaveshownthattheS-proteinisthemajorhemaggluti-sameeffectwasobservedafterincubationwiththeninofthes
8、evirusesrecognizingN-acetyl-9-O-acetyl-purifiedHNproteinfromNewcastlediseasevirus,neuraminicacid(Neu5,9Ac,)asareceptordeterm
