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1、J.gen.Virol.(I975),28,381-39o381PrintedinGreatBritainHaemagglutinationbyAvianInfectiousBronchitisVirus-aCoronavirusByR.W.BINGHAM,M.HILARYMADGEANDD.A.J.TYRRELLDivisionofCommunicableDiseases,ClinicalResearchCentre,Harrow,MiddlesexHAI3UJ,U.K.(Accepted5MayI975)SU
2、MMARYThehaemagglutinafingabilityofthreestrainsofIBVwasinvestigated.ItwasshownthatwhereasstrainBeaudettehadnodetectablehaemagglutinin,bothConnecticutandMassachusettsagglutinatedredcellsofvariousspecies.ThehaemagglutininofConnecticutwasdetectableaftersucrosegra
3、dientpurificationwhereasthatofMassachusettsrequiredboththepurificationstepandincubationwiththeenzymephospholipaseCtorevealit.Theagglutinationcouldbeinhibitedbyspecificantisera.Somestudiesonthenatureoftheredcellreceptor,andthepossiblepresenceofareceptordestroy
4、ingenzyme,arereported.INTRODUCTIONCoronavirusesaregroupedtogetheronthegroundsoftheirmorphology,etherlabilityandapparentRNAcontent(Tyrrelletal.I968).Otherwisetheyarerathernon-uniformandsofaronlyhaemagglutinatingencephalitisvirusofpigs(HEV)andcertainstrainsofhu
5、maneoronavirus,namelyOC38andOC43,havebeenshowntohaemagglutinate.Bothagglu-tinateredcellsofseveralspecies(McIntosh,1974).Therehavealsobeenreportsthatavianinfectiousbronchitisvirus(IBV)agglutinatedchickenredcells,althoughonlyaftertheviruspreparationhadbeenpre-t
6、reatedwithtrypsin(Corbo&Cunningham,I959)-thisagglu-tinincouldapparentlybeseparatedfromthevirusparticle(Biswal,Nazerian&Cunningham,1966)andwasnotspecificallyinhibitedbyimmuneserum.ItwasobservedonlywiththeMassachusettsstrainandothershavehaddifficultyinrepeating
7、theobservations.InthispaperwedescribehaemagglutinationwhichisapparentlyduetotheIBVparticle.METHODSVirusstrainsandpurification.Thevirusstrainsused,theirgrowthandpurificationhavebeendescribedpreviously(Bingham,1975).Antisera.ChickenimmuneseraagainstIBV-Massachu
8、settsandIBV-ConnecticutweresuppliedbyMrT.Hall,WellcomeResearchLaboratories,Beckenham,Kent.Controlchickennon-immuneserumwascollectedfromspecificpathogenfreechickens(WickhamLaboratories,Wic
