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1、高效液相色谱法快速测定葡萄干和葡萄酒中3种糠醛类物质汪辉1，黎瑛2，夏立新1，彭新凯1，李秀娟3，袁靖2（1.长沙市食品质量安全监督检测中心，长沙410013；2.湖南省食品安全生产工程技术研究中心，长沙410013；3.新疆吐鲁番地区质量与计量检测所，吐鲁番838000）摘要：建立高效液相色谱法快速测定葡萄干和葡萄酒中3种糠醛类物质。样品经甲醇-0.02mol/L醋酸铵（用冰醋酸调pH=3.5）（20+80）溶液超声提取后，离心，取上清液过微孔滤膜待分析。分析时流动相A为0.02mol/L醋酸铵（用冰醋酸调pH=3.5），B为甲醇，对目标化合物进行梯度洗
2、脱。流缺少样品提取净化步骤描述速为1.0mL/min，C18色谱柱分离，柱温为40℃，检测波长为285nm。5-羟甲基糠醛、糠醛、5-甲基糠醛在0.7-500mg/L浓度范围内线性良好，方法检出限分别为0.4,0.5,0.3mg/kg,回收率在90.36.8-109%之间，相对标准偏差在4.5925%以下。该方法前处理过程简单，快速、准确，适于同时快速测定葡萄干和葡萄酒中3种糠醛类物质。关键词：高效液相色谱法；葡萄干；葡萄酒；5-羟甲基糠醛；糠醛；5-甲基糠醛中图分类号：文献标志码：文章编号：RapidDeterminationofThreeFurfura
3、lsinRaisinandWinebyHighPerformanceLiquidChromatographyWANGHui1,LIYing2,XIALi-xin1,PENGXin-kai1,LIXiu-juan3,YUANJing2(1.ChangshaCenterofSupervisionandInspectiononFoodQualitySafety,Changsha410013China;2.FoodsafetyproductionEngineeringResearchCenterofHunanProvinceChangsha410013,China
4、;3.XinjiangTurpanareatestinginstituteofqualityandmetrology,Tulufan838000,China)Abstract:Amethodofhighperformanceliquidchromatographywasdevelopedforrapiddeterminationofthreefurfuralsinraisinandwine.Thesampleswereextractedbythesolutionof0.02mol/Lammoniumacetate(pH=3.5)usingultrasoni
5、c,then,centrifuged.Afterfilteredthrough0.45μmfilters,thefiltratewouldbeanalyzedbyhighperformanceliquidchromatography.MobilephaseAwas0.02mol/Lammoniumacetate(pH=3.5)andthemobilephaseBwasmethanolwithcurrentvelocityof1.0mL/min.Undergradientelution,thetargetcompoundswereseparatedbyC18
6、columnat40℃whichweredetectedon285nm.Thecalibrationcurvesshowedgoodlinearityfor5-Hydroxymethylfurfuralhydroxymethylfurfural,furfuraland5-Methylfurfuralmethylfurfuralintherangeof0.7-500mg/L.Thelimitsofdetectionofthreetargetcompoundswere0.4,0.5,0.3mg/kg,respectively.Thespikedrecoveri
7、esrangedfrom90.3%to109%withRSDslessthan4.59%.Thisproposedmethodwassimplepretreatment,rapidandaccuratewhichwassuitablefordeterminationofthreefurfuralsinraisinandwine.Keyword:Highperformanceliquidchromatography;Raisin;Wine;5-Hydroxymethylfurfural;Furfural;5-Methylfurfural收稿日期：2013-0
8、5-17、修订日期：2013-06-16基金项目：湖南省科技计划重
