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1、反义核酸抑制RON基因表达对A549的体外作用第l4卷第24期2004年12月中国现代医学杂志ChinaJournalofModemMedicineVo1.14No.24Dec.2oal4文章编号:1005-8982(2004)24—0001—05InVitroinhibitionofanti---sensenucleicacidofoncogeneRONonlungcancercelllineA549PANTie-cheng,XULi-jun,LIJun,ZHAOJin—ping,CHENTao,ZHANGNi,WEIXiang,PA
2、NYou—min,LIULi—gang(DepartmentofCardiothroracicSurgery,TondiHospital,Ton~iMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan,Hubei430030,P.R.China)?论着?Abstract:Objective:ToinvestigatetheeffectofInVitroinhibitionofRONgeneexpressionandcellgrowtheffectoflungcance
3、rcellhneA549,anti-sensenucleicacidoftransmembranedomainofRONgene(RONm)wasintroducedintothiscellline.Thepossibilityoftheanti-sensonucleicacidofgeneR0NasacandidateforlungcancergenetherapyWasalsoexplored.Methods:RONmcDNAWasinverselyinsertedineukaryoficexpressionvectorpcDNA3
4、.1.thantheanti—sensenucleicacidofthisdomainWastransferredintothestudiedceils.TheexpressionofRONgeneinthisexperimentandcontrolgroupsweremonitoredbyEUSAandthealterationofcellproliferationWascheckedbyM,ITrassayetc.Results:TheexpressionlevelofRONgeneinthetransfectedlungcance
5、rcellsWasreducedandthecellproliferationWasinhibitedcomparingwiththecontrolcelllines.Conclusion:Theanti—sensenucleicacidofRONmsignificantlyreducestheexpressignofRONgeneinthehighlyexpressedlungcancercellfineA549andmightinhibitthegrowthofthiscellline.Keywords:GeneRON;anti——
6、sensenucleicacid;anti.—sense;lungcancer,restrainCLCnumber:R733.7Documentcode:A反义核酸抑制RON基因表达对A549的体外作用潘铁成,徐利军,李军,赵金平,陈涛,张霓,魏翔,潘友民,刘立刚(华中科技大学同济医学院附属同济医院胸心外科,湖北武汉430030)摘要:目的探讨针对人RoN基因跨膜区段(RONm)反义核酸对肺癌细胞系A549活性和细胞增殖的抑制作用,研究以RONm为靶的肺癌基因治疗的可能性?方法将人RONmcDNA反向插入到真核表达栽体pcDNA3.1中
7、,转染肺癌细胞A549,利用ELISA检测细胞模型RON基因表达水平,同时利用MTT和细胞计数方法监测细胞生物学活性的变化.结果转染后的肺癌细胞RON基因表达量和细胞活性明显降低,并出现明显的细胞凋亡?结论RONm反义核酸明显抑制RON基因的表达,同时对肺癌细胞的生长有抑制作用,促进细胞凋亡?关键词:R0N基因;反义核酸;反义;肺癌;抑制中图分类号:R733.7文献标识码:ARON(RecepteurdOrigineNantais)gene,aMETproto—oncogenefamilymember,encodingareceptor
8、tyrosinekinaseRON—RTK,hasbeenimplicatedintheprogressionofcertainepithelialcancers.RON—RTKwasfoundwidely
