analysis of genetic variability and population structure of the endemic medicinal

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1、Gene520(2013)189–193ContentslistsavailableatSciVerseScienceDirectGenejournalhomepage:www.elsevier.com/locate/geneShortCommunicationAnalysisofgeneticvariabilityandpopulationstructureoftheendemicmedicinalLimoniumsinenseusingmolecularmarkersab,⁎,YuYanqiubaaDingGe,ZhangDaizhen,ZhaoLingling,ZhangBeibeia

2、ChemicalandBiologicalEngineeringCollege,YanchengInstituteofTechnology,Jiangsu,Yancheng224003,ChinabJiangsuProvincialKeyLaboratoryofCoastalWetlandBioresourcesandEnvironmentalProtection,YanchengNormalUniversity,Jiangsu,Yancheng224051,ChinaarticleinfoabstractArticlehistory:Limoniumsinenseisanendemicme

3、dicinalherbusedtotreatfever,hemorrhageandotherdisorders.IntheAccepted7March2013presentstudy,populationgeneticdiversitywaselucidatedusingrandomamplifiedpolymorphicDNAAvailableonline16March2013(RAPD),intersimplesequencerepeat(ISSR)andamplifiedfragmentlengthpolymorphism(AFLP)primers.Percentageofpolymorp

4、hicbands,Nei'sgenediversityandShannon'sinformationindexrevealedahighKeywords:levelofgeneticdiversityatspecieslevel.Theanalysisofmolecularvariancerevealedthat69.88%(RAPD),Limoniumsinense71.19%(ISSR)and70.97%(AFLP)ofvariabilitywerepartitionedamongindividualswithinpopulations,RAPDISSRwhichindicatedthe

5、coherenttrendbyGst(0.3849/0.3577/0.3670).Geneflownumber(Nm)was0.581/AFLP0.618/0.612,whichindicatedthattherewasalimitedgeneexchangebetweenpopulations.TheUPGMAclus-Geneticvariabilityteringresultsshowedthatthegeneticdistancehadnosignificantcorrelationwithgeographicdistance.TheseConservationresultsindica

6、tethatthesemarkerswerereliabletoolsforthedifferentiationanddeterminationofthegeneticdiversityamongthepopulationsofL.sinenseandtheconservationofexistingnaturalpopulationisnecessary.©2013ElsevierB.V.Allrightsreserved.1.Introduction(AFLP)isaDNAfingerprintingtechniquethatapproachestheidealmarkersystemfo

7、rresolvinggeneticdiversityamongindividuals,popu-Limoniumsinense,aperennialherbofthefamilyPlumbaginaceae,lationsandspecies(Jianetal.,2006;MuellerandWolfenbarger,1999).iswidelydispersedinsalineregionsthrougho