issr-pcr tool for discrimination and genetic structure analysis of

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1、MolecularPhylogeneticsandEvolution43(2007)240–250www.elsevier.com/locate/ympevISSR-PCR:ToolfordiscriminationandgeneticstructureanalysisofqPlutellaxylostellapopulationsnativetodifferentgeographicalareasaababa,*O.Roux,M.Gevrey,L.Arvanitakis,C.Gers,D.Bordat,L.LegalaLaboratoireDynamiquedelaBiodivers

2、ite´,Universite´PaulSabatierToulouseIII,UMR-CNRS5172,Bat4R3,31062Toulousecedex9,FrancebCIRAD,UPRHorticulture,TA40/L,CSIROCampusinternationaldeBaillarguet,34398MontpellierCedex5,FranceReceived25March2006;revised31August2006;accepted27September2006Availableonline7October2006AbstractThediamondback

3、moth(DBM),Plutellaxylostella(L.)isconsideredasthemostdestructivepestofBrassicaceaecropsworld-wide.Itsmigratorycapacitiesanddevelopmentofinsecticideresistanceinmanypopulationsleadstomoredifficultiesforpopulationmanage-ment.Tocontrolmovementofpopulationsandapparitionsofresistancecarriedbyresistantm

4、igrantindividuals,populationsmustbeidentifiedusinggeneticmarkers.Here,sevendifferentISSRmarkershavebeentestedasatoolforpopulationdiscriminationandgeneticvariationsamong19DBMpopulationsfromCanada,USA,Brazil,MartiniqueIsland,France,Romania,Austria,Uzbekistan,Egypt,Benin,SouthAfrica,Re´unionIsland,H

5、ongKong,Laos,JapanandfourlocalitiesinAustraliawereassessed.Twoclassificationmethodsweretestedandcompared:acommonmethodofgeneticdistanceanalysesandanovelmethodbasedonanadvancedstatisticalmethodoftheArtificialNeuralNetworks’family,theSelf-OrganizingMap(SOM).The188lociselectedrevealedaveryhighvariab

6、ilitybetweenpopulationswithatotalpolymorphismof100%andaglobalcoefficientofgenedifferentiationestimatedbytheNei’sindex(Gst)of0.238.Nevertheless,thelargestpartofvariabilitywasexpressedamongindividualswithinpopulations(AMOVA:73.71%andmeanpolymorphismof94%withinpopulations).Geneticdifferentiationamongt

7、heDBMpopulationsdidnotreflectgeographicaldistancesbetweenthem.Thetwoclassificationmethodshavegivenexcellentresultswithlessthan1.3%ofmisclassifiedindividuals.Theoriginofthehighgeneticdifferentiationandefficiencyofthetwoclassificationmetho