analysis of genetic variability in aristaeomorpha foliacea (crustacea,

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1、C.R.Biologies334(2011)705–712ContentslistsavailableatScienceDirectComptesRendusBiologieswww.sciencedirect.comGenetics/Ge´ne´tiqueAnalysisofgeneticvariabilityinAristaeomorphafoliacea(Crustacea,Aristeidae)usingDNA-ISSR(InterSimpleSequenceRepeats)markersa,bcbMariaVictoriaFerna´ndez

2、,FerruccioMaltagliati,FedericaG.Pannacciulli,a,*MariaIne´sRolda´naLaboratorid’IctiologiaGene`tica,UniversitatdeGirona,CampusdeMontilivi,17071Girona,SpainbMarineEnvironmentResearchCentre,ENEA–S.Teresa,POBox224,19100LaSpezia,ItalycDipartimentodiBiologia,Universita`diPisa,ViaDerna1

3、,56126Pisa,ItalyARTICLEINFOABSTRACTArticlehistory:ThisworkreportsthefirstgeneticdataofAristaeomorphafoliacea,amarinedecapodofReceived30April2011highcommercialvalue,fromsixMediterraneanlocalitiesandonenewfishinggroundinAcceptedafterrevision12July2011theAvailableMozambiqueChannel.Th

4、euseoffiveInterSimpleSequenceRepeat(ISSR)primersonline7September2011provided150polymorphicloci.Averageestimatesofgeneticdiversitydidnotsignificantlydifferamongsampledlocalities,withameanvalueofheterozygosityH=0.1050.015.Keywords:Analysisofmolecularvariance(AMOVA)allocated>98%ofge

5、neticvariabilitytothewithin-MarinedecapodsampleAristaeomorphacomponent,displayingvalueshigherthanthosepreviouslyreportedinISSRstudiesonfoliaceamarineGeneticinvertebrates.Clusteranalysesdidnotdetectgeographicallyorgeneticallydistinctvariabilitygroups.GeneTheobservedlackoflarge-sc

6、alegeneticdifferentiationisdiscussedinrelationtotheflowhighDNA-ISSRspotentialforlarvaldispersalofthespeciesandtofeaturesofthemarkeremployed.ß2011Acade´miedessciences.PublishedbyElsevierMassonSAS.Allrightsreserved.1.IntroductionA.foliacea,inordertogiveaninsightintogeneticvariabili

7、tyandstructureofthespecies.ThemoleculartechniqueofThegiantredshrimp,Aristaeomorphafoliacea(RissoISSRsreliesontheamplificationofDNAfragments1827),isanimportanteconomicresourceexploitedinthebetweentwocloselyandinverselyorientedmicrosatellitesMediterraneanSeasince1959[1].Recently,an

8、ewfishing[8].ISSRsdonotrequireanypreviousknowled