肾素抑制剂阿利吉仑对阿霉素肾病小鼠肾损伤及足细胞nephrin表达的影响.pdf

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1、第35卷第5期西安交通大学学报(医学版)Vo1．35No．52014年9月JournalofXi’anJiaotongUniversity(MedicalSciences)Sep．2014◇临床研究◇肾素抑制剂阿利吉仑对阿霉素肾病小鼠肾损伤及足细胞nephrin表达的影响伍巧源。，廖蕴华，杨桢华，郭谊。，周颖川，陈一强(广西医科大学：1．第一附属医院。肾内科，2．研究生院，3．第一附属医院检验科，4．第一附属医院病理科，5．第一附属医院呼吸科，广西南宁530021)摘要：目的观察肾素抑制剂阿利吉仑对阿霉素肾病小鼠肾损伤的保护作用及对

2、足细胞nephrin表达的影响。方法通过尾静脉注射阿霉素建立小鼠阿霉素肾病模型后给予阿利吉仑，另设正常对照组及阿霉素肾病对照组；比较不同组别尿蛋白及肾功能的差异；。肾组织石蜡切片行PAs、HE和Masson染色，观察各组肾小球硬化程度和。肾小管损害程度。RT-PCR及Westernblot检测各组nephrin的表达。结果阿霉素肾病小鼠表现为蛋白尿、低蛋白血症并伴有进行性肾功能下降，病理表现为肾小球硬化及小管间质损害；阿利吉仑可使蛋白尿状况显著改善，血清白蛋白上升，肾功能恶化得以延缓(P

3、改善(P

4、aliskirenonadriamycinnephropathyandnephrinexpressioninpodocytesinmiceWUQiao—yuan一，LIAOYun—hua，YANGZhen—hua，GUOYi。，ZHOUYing—Chuan。CHENYi—qiang(1．DepartmentofNephrology，theFirstAffiliatedHospitalofGuangxiMedicalUniversity，2．GraduateSchool，GuangxiMedicalUniversity，3．Depar

5、tmentofClinicalLaboratory，theFirstAffiliatedHospitalofGuangxiMedicalUniversity，4．DepartmentofPathology，theFirstAffiliatedHospitalofGuangxiMedicalUniversity，5．InstituteofRespiratoryDisease，theFirstAffiliatedHospitalofGuangxiMedica1University，Nanning530021，China)ABSTRACT

6、：ObjectiveTostudytheeffectsofrenininhibitoraliskirenonadriamycinnephropathyandnephrinexpressioninpodocytesinmice．MethodsMaleBALB／cmiceweresubjectedtoasingletailveininjectionofadriamycintoestablishthemodelofadriamycinnephropathy．Thenmiceweretreatedwithaliskiren；24一houru

7、rinesampleswerecollectedformeasurementofurinaryprotein．Miceweresacrificedatweek6．Theparaffin—embeddedsectionsoftheirkidneyswerestainedbyPAS，MassonandHEstaining．TheexpressionsofnephrinmRNAandproteinweredeterminedbyRT-PCRandWesternblot．ResultsAdriamycinnephropathyinmicew

8、ascharacterizedbyproteinuria，increasedserumcreatinine，progressiveglomerulosclerosis，andtubulointerstitiallesions．Alis