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1、中国组织工程研究与临床康复第14卷第32期2010–08–06出版JournalofClinicalRehabilitativeTissueEngineeringResearchAugust6,2010Vol.14,No.32骨髓间充质干细胞对阿霉素肾病大鼠肾脏nephrin表达的影响*★董晨，杨焕丹，丰炳峰，关凤军，赵彤Effectsofbonemarrowmesenchymalstemcellsonnephrinexpressioninthekidneyofadriamycin-inducednephriticratsDongChen,YangHuan-dan,FengBin
2、g-feng,GuanFeng-jun,ZhaoTongAbstractDepartmentofBACKGROUND:Numerousstudieshaveaddressedbonemarrowmesenchymalstemcells(BMSCs)transplantationfortreatingPediatrics,Hospitalacutekidneyinjury,butfewreportshaveconcernedBMSCstransplantationforchronickidneydisease.AffiliatedtoXuzhouOBJECTIVE:Toinvest
3、igatetherepaireffectsofBMSCstransplantationonpodocytesofadriamycin-inducednephroticratsviaMedicalCollege,Xuzhou221000,abdominalaortaandeffectsonNephrinmRNAexpressioninthekidney.JiangsuProvince,METHODS:BMSCsofSpragueDawleyratswereharvested,culturedandpurifiedbywholebonemarrowmethod+adherenceCh
4、inascreeningmethod,andthenusedfortransplantation.TheSpragueDawleyratswererandomlyassignedtothreegroups:normalgroup:normalSpragueDawleyrats,nointervention;modelgroup:injectionofadriamycinonceintocaudalvein5mg/kg+DongChen★,infusionofsaline1mLintoabdominalaortaonthesameday;BMSCsgroup:injectionof
5、adriamycinonceintocaudalveinMaster,Chief7physician,5mg/kg+infusionofstemcells1×10intoabdominalaortaonthesameday,about1mLvolume.EightratsfromeachgroupDepartmentofwerecollectedat7,14and28daysfollowingtreatment.Thecellmembraneantigensweredetectedwithflowcytometryanalysis.Pediatrics,HospitalTheur
6、ineproteinwasmeasuredat24hoursusingsulfosalicylicacid-sodiumsulfateturbidimetricmethod.SerumalbuminandAffiliatedtoXuzhoubloodfatwereexaminedutilizingautomaticbiochemistryanalyzer.TherenalhistologicalchangeswereobservedunderMedicalCollege,transmissionelectronmicroscopeandusinghematoxylin-eosin
7、staining.TheexpressionofNephrinmRNAinthekidneytissuesXuzhou221000,JiangsuProvince,wasmeasuredusingreversetranscription-polymerasechainreaction.ChinaRESULTSANDCONCLUSION:Comparedwithnormalgroup,ratsinmodelgroupdevelopednephropathycharacterized
