MGMT基因启动子甲基化检测在脑胶质瘤化疗中的意义_郑长青.pdf

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1、《癌症》ChineseJournalofCancer，2009，28（6）：575－580575·基础研究·MGMT基因启动子甲基化检测在脑胶质瘤化疗中的意义郑长青111221，季守平，宫锋，李安民，邰军利，章扬培6DetectionofO-methylguanine-DNAmethyltransferasepromotermethylationinchemotherapyforglioma111221Chang-QingZheng，Shou-PingJi，FengGong，An-MingLi，Jun-LiTaiandYang-PeiZhang［Abstr

2、act］BackgroundandObjective：EpigeneticsilencingoftheDNArepairgene6，O-methylguanine-DNAmethyltransferase（MGMT），isassociatedwiththetherapeuticresponsetomethylatingagents．ThisstudywastoassessthevalueofdetectingthepromotermethylationofMGMTgeneinchemotherapyforglioma．Methods：Methylation

3、-specificPCR（MSP）wasemployedtodetectMGMTpromoterCpGislandmethylationin39samplesofgliomatakenfromsurgery．Westernblotandimmunohistochemistrywereusedtodetect1．军事医学科学院野战输血研究所，proteinexpression．MTTwereemployedtodetectthesensitivityoftwoglioma血液生物化学与分子生物学实验室，celllinestoalkylatingagents，

4、ACNUandTMZ．TheKaplan-Meiercurvewas北京100850adoptedtoestimatetheoverallsurvivalaccordingtothemethylationstatusof2．解放军总医院第一附属医院theMGMTpromoter．Results：MethylationofMGMTpromoterCpGislandwas脑外科，detectablein46．2％ofgliomatissues，butnotinanynormaltissues．The北京100037expressionrateofMGMTpro

5、teinwas61．5％．ThestatusofMGMTmethylationstatuswasassociationwiththeproteinlevelofMGMT（P＜0．05）．TheMGMT1．DepartmentofBiochemistryandgenewasdemethylatedingliomacelllineSHG-44following5-Aza-CdRMolecularBiology，treatment；theexpressionofMGMTproteinwasrestoredandtheresistanceInstituteofTr

6、ansfusionMedicine，ofSHG44cellstoalkylatingagentswasreversed．TheoverallsurvivalwasAcademyofMilitaryMedicalSciences，higherinpatientswithmethylatedMGMTpromoterthaninthosewithBeijing，100850，unmethylatedMGMTpromoter（P＜0．05）．Conclusions：ThestatusofMGMTP．R．ChinapromoterCpGislandmethylati

7、oniscloselycorrelatedtoMGMTprotein2．TheFirstAffiliatedHospital，expressionandsensitivityofcellstoalkylatingagentsinglioma．DetectionofPLAGeneralHospital，themethylatedsequencesofMGMTmaybeusedasapredictivefactorforBeijing，100037，thetreatmentofgiloma．P．R．ChinaKeywords：glioma，promoterCp

8、Gisland，hypermethyaltion，DNArepai