间充质祖细胞源性神经元样细胞肌内移植延缓骨骼肌萎缩.pdf

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1、Science&TechnologyVision项目钠科技视界科技·探索·争鸣问充质祖细胞源性神经元样细胞肌内移植延缓骨骼肌萎缩王丽娟赵文勇2(1．西南药业股份有限公司，中国重庆400038；2．重庆市公安局九龙坡区分局，中国重庆400039)【摘要】目的：探讨间充质祖细胞(Mesenchymalprogenitorcell，MPC)源性神经元样细胞移植于靶肌肉内延缓失神经}生骨骼肌萎缩。A-法：取GFP转基因C57小鼠后肢长骨进行MPC培养、诱导及鉴定。选取C57小鼠36只，随机分为MPC移植组、神经断离组及对照组，MPC移植组腓肠肌内散

2、点注入5lxLMPC悬液，神经断离组腓肠肌对应部位散点注入等量PBS，对照组不作处理。观察小鼠后肢活动能力，术后2和4周测量腓肠肌湿重、肌纤维横截面积维持率及观察超微结构，用Westernblot检测d～actin、MHC及RT—PCRk2~'lMyogenin。MyoD的表达。结果：术后2和4周．MPC移植组腓肠肌湿重及肌纤维横截面积维持率显著高于神经断离组(P

3、P

4、qingJiulongpoDistrictPublicSecurityBureau，Chongqing400039，China)【Abstract]Objective：Tostudythedelayingdenervatedskeletalmuscleatrophyaftertransplantationofneuron-likecellsfrommesenchymalDrogenitorcell(MPC)intothetargetmuscle．．Methods：MPCwereisolatedfrombonesofhindlimbsofGF

5、PtransgenicC57miceforcultivation，induetion．andidentification．36C57miceweredividedinto3groupsevenlyinrandom，MPCtransplantationgroup，thetransectedgroupandthec0ntrolgroup．5LofMPCsuspensionand51xLofPhosphatebufferedsaline(PBS)wereinjectedintothegastrocnemiusintheMPCtransplanta

6、tiongroupandthetransectedgrouprespectivelywhilenothingwasinjectedinthecontrolgroup．Theactivityabilityofhindlimbsofmicewereobserved．Atthetimepointof2and4weeksaftertheoperation，theretainratioofwetweightofgastroenemiusmuscleandcrosssectionalareaofmusclefiberwasmeasuredandtheu

7、ltrastructuralorganizationwasobserved．Theexpressionof0【一aetin，myoglobulin(MHC)weredetectedbywesternblotandtheexpressionofMyogeninandMyoDweredetectedbyRT—PCR．Results：Atthetimepointof2and4weeksaftertheoperationthewetweightofgastrocnemiusmuscleandtheretainratioofcrosssectiona

8、lareaofmusclefiberofmiceoftheMPCtransplantationgroupwashigherthanthatofthetranseetedgroup