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时间:2020-05-15
《间充质祖细胞源性神经元样细胞肌内移植延缓骨骼肌萎缩.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、Science&TechnologyVision项目钠科技视界科技·探索·争鸣问充质祖细胞源性神经元样细胞肌内移植延缓骨骼肌萎缩王丽娟赵文勇2(1.西南药业股份有限公司,中国重庆400038;2.重庆市公安局九龙坡区分局,中国重庆400039)【摘要】目的:探讨间充质祖细胞(Mesenchymalprogenitorcell,MPC)源性神经元样细胞移植于靶肌肉内延缓失神经}生骨骼肌萎缩。A-法:取GFP转基因C57小鼠后肢长骨进行MPC培养、诱导及鉴定。选取C57小鼠36只,随机分为MPC移植组、神经断离组及对照组,MPC移植组腓肠肌内散
2、点注入5lxLMPC悬液,神经断离组腓肠肌对应部位散点注入等量PBS,对照组不作处理。观察小鼠后肢活动能力,术后2和4周测量腓肠肌湿重、肌纤维横截面积维持率及观察超微结构,用Westernblot检测d~actin、MHC及RT—PCRk2~'lMyogenin。MyoD的表达。结果:术后2和4周.MPC移植组腓肠肌湿重及肌纤维横截面积维持率显著高于神经断离组(P3、P4、qingJiulongpoDistrictPublicSecurityBureau,Chongqing400039,China)【Abstract]Objective:Tostudythedelayingdenervatedskeletalmuscleatrophyaftertransplantationofneuron-likecellsfrommesenchymalDrogenitorcell(MPC)intothetargetmuscle..Methods:MPCwereisolatedfrombonesofhindlimbsofGF5、PtransgenicC57miceforcultivation,induetion.andidentification.36C57miceweredividedinto3groupsevenlyinrandom,MPCtransplantationgroup,thetransectedgroupandthec0ntrolgroup.5LofMPCsuspensionand51xLofPhosphatebufferedsaline(PBS)wereinjectedintothegastrocnemiusintheMPCtransplanta6、tiongroupandthetransectedgrouprespectivelywhilenothingwasinjectedinthecontrolgroup.Theactivityabilityofhindlimbsofmicewereobserved.Atthetimepointof2and4weeksaftertheoperation,theretainratioofwetweightofgastroenemiusmuscleandcrosssectionalareaofmusclefiberwasmeasuredandtheu7、ltrastructuralorganizationwasobserved.Theexpressionof0【一aetin,myoglobulin(MHC)weredetectedbywesternblotandtheexpressionofMyogeninandMyoDweredetectedbyRT—PCR.Results:Atthetimepointof2and4weeksaftertheoperationthewetweightofgastrocnemiusmuscleandtheretainratioofcrosssectiona8、lareaofmusclefiberofmiceoftheMPCtransplantationgroupwashigherthanthatofthetranseetedgroup
3、P4、qingJiulongpoDistrictPublicSecurityBureau,Chongqing400039,China)【Abstract]Objective:Tostudythedelayingdenervatedskeletalmuscleatrophyaftertransplantationofneuron-likecellsfrommesenchymalDrogenitorcell(MPC)intothetargetmuscle..Methods:MPCwereisolatedfrombonesofhindlimbsofGF5、PtransgenicC57miceforcultivation,induetion.andidentification.36C57miceweredividedinto3groupsevenlyinrandom,MPCtransplantationgroup,thetransectedgroupandthec0ntrolgroup.5LofMPCsuspensionand51xLofPhosphatebufferedsaline(PBS)wereinjectedintothegastrocnemiusintheMPCtransplanta6、tiongroupandthetransectedgrouprespectivelywhilenothingwasinjectedinthecontrolgroup.Theactivityabilityofhindlimbsofmicewereobserved.Atthetimepointof2and4weeksaftertheoperation,theretainratioofwetweightofgastroenemiusmuscleandcrosssectionalareaofmusclefiberwasmeasuredandtheu7、ltrastructuralorganizationwasobserved.Theexpressionof0【一aetin,myoglobulin(MHC)weredetectedbywesternblotandtheexpressionofMyogeninandMyoDweredetectedbyRT—PCR.Results:Atthetimepointof2and4weeksaftertheoperationthewetweightofgastrocnemiusmuscleandtheretainratioofcrosssectiona8、lareaofmusclefiberofmiceoftheMPCtransplantationgroupwashigherthanthatofthetranseetedgroup
4、qingJiulongpoDistrictPublicSecurityBureau,Chongqing400039,China)【Abstract]Objective:Tostudythedelayingdenervatedskeletalmuscleatrophyaftertransplantationofneuron-likecellsfrommesenchymalDrogenitorcell(MPC)intothetargetmuscle..Methods:MPCwereisolatedfrombonesofhindlimbsofGF
5、PtransgenicC57miceforcultivation,induetion.andidentification.36C57miceweredividedinto3groupsevenlyinrandom,MPCtransplantationgroup,thetransectedgroupandthec0ntrolgroup.5LofMPCsuspensionand51xLofPhosphatebufferedsaline(PBS)wereinjectedintothegastrocnemiusintheMPCtransplanta
6、tiongroupandthetransectedgrouprespectivelywhilenothingwasinjectedinthecontrolgroup.Theactivityabilityofhindlimbsofmicewereobserved.Atthetimepointof2and4weeksaftertheoperation,theretainratioofwetweightofgastroenemiusmuscleandcrosssectionalareaofmusclefiberwasmeasuredandtheu
7、ltrastructuralorganizationwasobserved.Theexpressionof0【一aetin,myoglobulin(MHC)weredetectedbywesternblotandtheexpressionofMyogeninandMyoDweredetectedbyRT—PCR.Results:Atthetimepointof2and4weeksaftertheoperationthewetweightofgastrocnemiusmuscleandtheretainratioofcrosssectiona
8、lareaofmusclefiberofmiceoftheMPCtransplantationgroupwashigherthanthatofthetranseetedgroup
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