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1、胎儿心脏黏附细胞具有类似间充质祖细胞特征作者：江小霞，苏永锋，李秀森，张毅，吴英，毛宁【摘要】 为了观察人心脏是否含具有间充质祖细胞特性的细胞，从胎儿心脏分离、培养单个核细胞并从形态、表型和功能3个方面与骨髓间充质祖细胞进行比较和鉴定。结果表明，从心脏分离培养的细胞为成纤维样，表面抗原为CD73,CD105,CD29,CD44,HLA-ABC,CD166阳性，而CD45,CD34,CD86,HLA-DR阴性。在不同的分化体系中，细胞能分化为脂肪细胞、成骨细胞和软骨细胞。细胞扩增迅速，具有低免疫原性特性。结论：从心脏分离培养的细胞具有间充质祖细胞特性。【关键词
2、】胎儿心脏；心脏黏附细胞；间充质祖细胞 HumanFetalHeart-derivedAdherentCellsilartoMesenchymalProgenitorCells AbstractThisstudyedtoinvestigateifhumanheartharboredapopulationofprimitiveundifferentiatedcellshumanfetalheartandarroorphology,phenotypesandfunctionsethodsdevelopedforMPCfromothersources.There
3、sultsshoorphologically,cellsbledfibroblasts.InthEirundifferentiatedstate,cellsedia,cellsdifferentiatedintoadipocytes,osteocytesandchondrocytesrespectively.TheycouldbeextensivelyexpandedinvitroandexhibitedverylomunogenicityasevaluatedbyTcellproliferationassays.Itisconcludedthatcellsi
4、solatedfromfetalheartpossesssimi-laritytothEIradultandfetalbonemarroorphologic,immunophenotypic,andfunctionalcharacteristics. Keyesenchymalprogenitorcell Bonemarroesenchymalprogenitorcells(MPC)haveattractedgreatattentionbecauseoftheircapabilityforreneesenchymaltissues［1］andtheirpo
5、tentialplatformsforthesystemicdeliveryoftherapeuticproteinsinvivofolloalmodelshaveshoarroigrateandengraftinnumerousorgansanddifferentiatealongtissue-specificlineagesunderthestimulationoflocalfactors,andmaybeusefulintherepairorregenerationofdamagedormutatedbone,cartilage,ormyocardial
6、tissues［3-5］.Recentanytissues,includingumbilicalcord［6］,umbilicalcordblood［7］,bonemarroentofacutemyocardialinfarctionhasbeeanoveltherapeuticoption,theknoited［9］.Ouraimanfetalheart. MaterialsandMethods Isolationandcultureofadultandfetalbonemar-roesenchymalprogenitorcellsTheResearch
7、EthicsmitteesofXuanantissueforresearchpurposes.Humanfetalheartsamplesaccidentalabortusof4.0-5.0monthsunderconsent.Single-cellsuspensionsoffetalheartmesenchymaltissueincingmyocardialtissue,athebloodvessel,througha70-μm-nylonfilter.Thencellsfromadultandfetalbonemarroplesandfetalhearts
8、amples(FBS;Hyclone,
