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1、胎儿心脏黏附细胞具有类似间充质祖细胞特征姓名：2014年6月25日胎儿心脏黏附细胞具有类似间充质祖细胞特征作者：江小霞，苏永锋，李秀森，张毅，吴英，毛宁【摘要】为了观察人心脏是否含具有间充质祖细胞特性的细胞，从胎儿心脏分离、培养单个核细胞并从形态、表型和功能3个方面与骨髓间充质祖细胞进行比较和鉴定。结果表明，从心脏分离培养的细胞为成纤维样，表面抗原为CD73,CD105,CD29,CD44,HLA-ABC,CD166阳性，而CD45,CD34,CD86,HLA-DR阴性。在不同的分化体系中，细胞能分化为脂肪细胞、成骨细胞和软骨细胞。细胞扩増迅
2、速，具有低免疫原性特性。结论：从心脏分离培养的细胞具有间充质祖细胞特性。【关键词】胎儿心脏；心脏黏附细胞；间充质祖细胞HumanFetalHeart-derivedAdherentCellswithCharacteristicsSimilartoMesenchymalProgenitorCellsAbstractThisstudywasaimedtoinvestigateifhumanheartharboredapopulationofprimitiveundifferentiatedcellswiththecharacteristicsof
3、MPC.Cellswereisolatedfromhumanfetalheartandwereculturedunderconditionsappropriateforbonemarrow-derivedMPCs.Theirmorphology，phenotypesandfunctionsweretestedbymethodsdevelopedforMPCfromothersources.Theresultsshowedthatmorphologically，cellswerespindleshapedandresembledfibrobla
4、sts.Intheirundifferentiatedstate,cellswereCD73,CD105,CD29,CD44,HLA-ABC,CD166positiveandCD45,CD34,CD86,HLA-DRnegative.Whenculturedinadipogenic，osteogenicorchondrogenicmedia，cellsdifferentiatedintoadipocytes，osteocytesandchondrocytesrespectively.Theycouldbeextensivelyexpanded
5、invitroandexhibitedverylowimmunogenicityasevaluatedbyTcellproliferationassays.Itisconcludedthatcellsisolatedfromfetalheartpossesssimi-laritytotheiradultandfetalbonemarrowcounterpartsinmorphologic,immunophenotypic，andfunctionalcharacteristics.Keywordsfetalheart;heartderiveda
6、dherentcell;mesenchymalprogenitorcellBonemarrow-derivedmesenchymalprogenitorcells(MPC)haveattractedgreatattentionbecauseoftheircapabilityforrenewalanddifferentiationintovariouslineagesofmesenchymaltissues[1]andtheirpotentialplatformsforthesystemicdeliveryoftherapeuticprotei
7、nsinvivofollowinggenetransferusingoncogenicretroviruses[2]•Studiesinvolvingavarietyofanimalmodelshaveshownthatadultbonemarrow-derivedMPCcanmigrateandengraftinnumerousorgansanddifferentiatealongtissue-specificlineagesunderthestimulationoflocalfactors，andmaybeusefulintherepai
8、rorregenerationofdamagedormutatedbone，cartilage，ormyocardialtissues[3-5].Recentwor
