资源描述:
《胎儿心脏黏附细胞具有类似间充质祖细胞特征论文》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、胎儿心脏黏附细胞具有类似间充质祖细胞特征论文江小霞,苏永锋,李秀森,张毅,吴英,毛宁【摘要】为了观察人心脏是否含具有间充质祖细胞特性的细胞,从胎儿心脏分离、培养单个核细胞并从形态、表型和功能3个方面与骨髓间充质祖细胞进行比较和鉴定。结果表明,从心脏分离培养的细胞为成纤维样.freelanFetalHeart-derivedAdherentCellsilartoMesenchymalProgenitorCellsAbstractThisstudyedtoinvestigateifhumanheartharboredapopulationofprimitiveundifferentiat
2、edcellshumanfetalheartandarroorphology,phenotypesandfunctionsethodsdevelopedforMPCfromothersources.Theresultsshoorphologically,cellsbledfibroblasts.Intheirundifferentiatedstate,cellsedia,cellsdifferentiatedintoadipocytes,osteocytesandchondrocytesrespectively.Theycouldbeextensivelyexpandedinvitr
3、oandexhibitedverylomunogenicityasevaluatedbyTcellproliferationassays.Itisconcludedthatcellsisolatedfromfetalheartpossesssimi-laritytotheiradultandfetalbonemarroorphologic,immunophenotypic,andfunctionalcharacteristics.KeyesenchymalprogenitorcellBonemarroesenchymalprogenitorcells(MPC)haveattracte
4、dgreatattentionbecauseoftheircapabilityforreneesenchymaltissues[1]andtheirpotentialplatformsforthesystemicdeliveryoftherapeuticproteinsinvivofolloalmodelshaveshoarroigrateandengraftinnumerousorgansanddifferentiatealongtissue-specificlineagesunderthestimulationoflocalfactors,andmaybeusefulinther
5、epairorregenerationofdamagedormutatedbone,cartilage,ormyocardialtissues[3-5].Recentanytissues,includingumbilicalcord[6],umbilicalcordblood[7],bonemarroentofacutemyocardialinfarctionhasbeeanoveltherapeuticoption,theknoited[9].Ouraimanfetalheart.MaterialsandMethodsIsolationandcultureofadultandfet
6、albonemar-roesenchymalprogenitorcellsTheResearchEthicsmitteesofXuanantissueforresearchpurposes.Humanfetalheartsamplesaccidentalabortusof4.0-5.0monthsunderconsent.Single-cellsuspensionsoffetalheartmesenchymaltissueincingmyocardialtissue,athebloodvessel,througha70-μm-nylonfilter.Thencellsfromadul
7、tandfetalbonemarroplesandfetalheartsamples(FBS;Hyclone,Logan,UT,USA)inDulbecco'sModifiedEagle'sMedium-Lo)lpenicillin,50μg/mlstreptomycin,and2mmol/LL-glutamine.Cells2andincubatedat37℃in5%CO2.After36hours,nonadherentcellsoved,andthe