诱导骨髓间充质干细胞向心肌样分化及其移植的可行性.pdf

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1、中国组织工程研究与临床康复秽73孝彩40筋‘2009—10—01出版JournalofClinicalRehabilitativeTissueEngineeringResearchOctober7，2009V01．13，No．40微★◆血管内皮细胞诱导骨髓间充质干细胞向心肌样分化及其移植的可行性’’。’。廖礼强，张晓刚，史若飞，王丁琼，汤为学Roleofmicrovascularendothelialcellsininducingbonemarrowmesenchymalstemcellsdiferentiationintocardiomyocytesandimplant

2、ationfeasibility’’。’。LiaoLi—anZhangXiao-anShiRuo-feiWangDing—onTangWei．xueqig，gg，，qig，Abstract’DepartmentofBACKGROUND：Bonemarrowmesenchymalstemcells(BMSCs)haveanenormouspotentialonmyocardium—injureddiseasesCardiologYHrstAffiliatedHospital．viacardiomyogenesis．however．an}deaIjnducingmethodh

3、asnotbeenfoundatpresent．ChongqingMedicalOBJECTIVE：Toinvestigatethepotentialofratmicrovascularendothelialcells(mECs)oninducingBMSCsdiferentiationjntoUniversity．cardiomyocytes，andtoanalyzethefeasibilityoftransplantation．ChongqingDESIGN．TIMEANDSETTING：AnobservationaIexperimentbasedoncellsand

4、animalswasconductedattheChongqingKey400016．China；‘ChongqingLaboratoryofNeurologybetweenJuly2008andFebruary2009．EmergencyMedicalMATERlALS：AtotaIof16clearhealthymaleSpragueDawleyratsaged2—3monthswereobtainedfrOmtheExperimentalCenter，ChongqingAnimaICenter．ChongqingMedicaIUniversity．400016．Ch

5、ina；。METHODS：Transwellcabinof0．4umborewasplacedincultureplatewelItoconstructdoubl~cabinculturesystem．BMSCsChongqingKeyLaboratoryofandmECswererespectivelyisolatedandculturedfrom1SpragueDawleyratusingFicolIdensitygradient+adherenceNeurology．screeningmethodandexplantmethod．mECswerewelfcultur

6、edandreached50％confluency．ThemediumwaschangedeveryChongqing3days．Followingcollectionandfiltration，theconditionedmediumwasobtainedInv#roexperimentwasdividedintodirect400016．Chinacontactrou-chambercoculturerouconditionedmediumrouandmediumcontrolroup(IV)andgp(I)，bigp(1I)，gp(111)g，-LiaoLi-ank

7、mocardiaIinductionefectwasobservedintheseconditionsraratswerecommittedtoqig，y．AtotaIof15healthySpgueDawley’Studyingformasters=n=6coronaryligationformyocardialinfarctionmodelsanddividedintocellimplantationgroup(n8)andmodelcontrolgroup()．1degree．Physician．。weekfoIlowi