针刺对背根节神经元GDNF表达的影响.pdf

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1、四川大学学报(医学版D;4(D:45~47JSichuanUniV(MedSciEdiD针刺对背根节神经元GDNF表达的影响袁源1王廷华1杨志敏2刘芬11.昆明医学院神经科学研究所(昆明650031D;2.昆明医学院第一附属医院神经外科[摘要)目的研究部分去背根及针刺对背根神经节神经元胶质细胞源性神经营养因子(GDNFD表达的影响O方法制作猫备用背根模型并针刺备用根支配区的两组穴位足三里和悬钟伏兔和三阴交O针刺7d后取备用背根节用GDNF抗血清(1=1000D行免疫组织化学染色O分别计数各组大~中~小阳性神经元的数量O结果非手术组GDNF阳性神

2、经元数量为55%其中大神经元为25%中神经元为14%小神经元为16%;术后非针刺组阳性神经元总数为35%较非手术组明显下降(P<0.05D主要为大神经元(5%D数量减少而阳性中(13%D~小神经元(17%D数量变化不明显但染色明显加深O针刺组GDNF阳性神经元数量又明显增加至90%其中阳性大神经元2S%中神经元17%小神经元45%O结论背根节GDNF阳性大神经元数量在去部分背根术后7d显著下降而针刺可逆转这种变化OGDNF阳性中~小神经元数量在术后7d并无明显变化但针刺后表达显著增加O[关键词)GDNF针刺备用背根节[中图分类号)R651Exp

3、ressionofGDNFinDorsalRootGanglionafterPartialDorsalRootRhizotomyandAcupunctureinSparedRoot.YuonYuonWongtinghuoYongzhiminLiuFen.InstituteofNeu7osieneKunmingMediclCollegeKunming650031China[Obstract)ObjectiVeInvestigatingtheexpressionofglialcellline-derivedneurotrophicfactor(GD

4、NFDindorsalrootganglionafterpartialdorsalrootrhizotomyandacupunctureinsparedroot.MethodsFifteenadultcatsweredividedintothreegroups.Twogroupsweresubjectedtounilateralsparedrootrhizotomy(L1-L5L7-S2DRGsweresectionedL6DRGwassparedD.Inoneoftheseoperatinggroupstwosetsofacupoints(Z

5、usanliandXuanzhong;FutuandSanyinjiaoDlocatedinthedistributionareaofspinalnerveL6wereelectro-stimulatedalternatively30mineverydaybyelectro-needling.Theotheroperationgroupwasnottreatedwithacupuncture.thOnthe7dayafterpartialdorsalrhizotomyandacupuncturetheanimalsofthesetwogroup

6、sandanothergroup(intactanimalsasreferencegroupDweresacrificed.TheDRGs(L6DweretakenandmadeintofrozensectionsandwerestainedunderthesameconditionusingspecificGDNFantibody(1=1000DbyABCmethod.TheGDNFpositivelargemediumandsmallsizedneuronsinDRGwerecounted.ResultsInintactanimalsabo

7、ut55%neuronsincludinglargesizedneurons(25%Dandafewsmall(16%Dandmediumsizedneurons(14%Dwerestained.Incontrastat7daysafterpartialdorsalrootrhizotomythenumbersofpositiveneuronsdecreasedgreatly(35%Dmorethanthatofreferencegroup(P<0.05D.~oweverfollowingacupunctureabout90%neuronsin

8、cludinglarge(2S%Dmedium(17%Dandsmall(45%Dneuronscametobestainedagain.Conclu