gdnf在慢性收缩性损伤大鼠脊髓背根神经节中的蛋白表达

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1、GDNF在慢性收缩性损伤大鼠脊髓背根神经节中的蛋白表达作者：夏小萍，马正良，朱巍，周锦勇【摘要】目的探讨胶质细胞源性神经营养因子(GDNF)在慢性收缩性损伤(CCI)所致神经病理性疼痛大鼠的L4背根神经节中的蛋白表达。方法实验1：24只SD大鼠，随机分为正常组(Naive组)、假手术组(Sham组)和CCI组(每组n=8)。Naive组不做任何手术;Sham组仅暴露坐骨神经;CCI组按Bennett法结扎右侧坐骨神经主干制备CCI模型。分别测定Sham组和CCI组术前2天(基础值)及术后第1、3、5、7、14、21天(Naive组

2、则在相对应的时间点)大鼠同侧(本实验为右侧)后足机械缩足反射阈值(MWT)和热缩足反射潜伏期(PWL)。实验2：18只SD大鼠随机分为正常组(Naive组)、假手术组(Sham组)和CCI组(每组n=6)。CCI组制备CCI模型，术后第7天处死大鼠，取同侧L4背根神经节;Naive组不做任何手术，直接取右侧L4背根神经节;Sham组仅暴露坐骨神经，术后第7天处死大鼠，直接取右侧L4背根神经节。采用Westernblot法检测脊髓L4背根神经节GDNF的蛋白表达。结果实验1：与Sham组相比，CCI组大鼠从术后第3天开始至术后21天

3、内各时间点MWT和PWL均显著降低(P<0.05或P<0.01)。实验2：与Sham组相比，CCI组在L4背根神经节的GDNF表达明显减少(P<0.01)。结论CCI大鼠的L4背根神经节的GDNF表达减少，可能参与CCI所致神经病理性疼痛的发病机制。9【关键词】慢性收缩性损伤;神经病理性疼痛;胶质细胞源性神经营养因子;背根神经节;动物模型;大鼠　　Abstract：ObjectiveToinvestigatetheexpressionofglialcellline-derivedneurotrophicfacto

4、r(GDNF)inthefourthlumbar(L4)dorsalrootganglion(DRG)inratswithneuropathicpaininducedbychronicconstrictiveinjury(CCI).MethodsExperiment1:24Sprague-DawleyratswererandomlydesignatedasNaivegroup,ShamgroupandCCIgroup(n=8each).TheCCImodelwasestablishedbylooseligationaroundth

5、erightL4sciaticnerveasdescribedbyBennett.IntheShamgrouponlytherightsciaticnervewasexposedandintheNaivegroupnotreatmentwasperformed.Mechanicalwithdrawalthreshold(MWT)usingVonFreyfilamentandpawwithdrawallatency(PWL)usingradiantheatappliedtotherighthindplantarsurfacewere

6、measured2daysbeforesurgery(baseline)and1,3,5,7,14and21daysafteroperation(withtheNaivegroupmeasuredatthecorrespondingtimepoints).Experiment2:18Sprague-DawleyratsweredividedintoNaive,ShamandCCIgroups(n=6each).TheanimalmodelwasestablishedwiththeCCIgroup.TheratsinCCIgroup

7、sweredecapitatedonthe7thdayafteroperation9andtherightL4DRGwasremoved;intheNaivegrouptheratsunderwentnooperationandtherightL4DRGwasdirectlyremoved,andintheShamgrouptherightsciaticnervewasonlyexposedandtherightL4DRGwastakenout.TheexpressionofGDNFofspinalL4DRGwasdetermin

8、edbyWesternblot.ResultsInExperiment1,MWTandPWLofratsintheCCIgroupweresignificantlylowerthanthoseintheShamgroupfromday3today2