sirna沉默卵巢癌cxcr4基因对卵巢癌腹腔转移的影响

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1、可北医药2013年12月第35卷第24期HebeiMedicalJournal，2013，Vol35DecNo．243701doi：10．3969／j．issn．1002—7386．2013．24．007·论著·siRNA沉默卵巢癌CXCR4基因对卵巢癌腹腔转移的影响燕霞燕锋张静【摘要】目的研究应用基因靶向治疗对卵巢癌腹腔转移的实验疗效观察。方法制作好的卵巢癌大鼠模型60只，采用随机数字表法随机分为实验组和对照组，实验组应用基因靶向治疗，对照组采用阴性治疗，siRNA沉默卵巢癌CXCR4基因后，观察CXCR4蛋白的表达情况，对卵巢

2、癌细胞增殖的影响，观察应用基因靶向治疗后腹腔转移情况，评价大鼠生存情况。结果实验组在治疗后腹水中的CXCR4显著减少(P<0．05)；对照组治疗后腹水中的CXCR4量显著性增加(P<0．05)；实验组与对照组相比，治疗前腹水中CXCR4，差异无统计学意义(P>0．05)；治疗后差异有统计学意义(P<0．05)。实验组腹水抽出量与实验组相比，明显减少(P<0．05)。结论应用基因靶向治疗方法siRNA沉默卵巢癌CXCR4基因对卵巢癌腹腔转移，具有较好的疗效，临床预后较好。【关键词】卵巢癌；腹腔转移；基因靶向治疗【中图分类号】R737

3、．31【文献标识码】A【文章编号】1002—7386(2013)24—3701—03EfectofsmallinterferingRNA—inducedCXCR4silencegeneonpe~toncalmetastasisofova~ancancerinratsYANX／a，YANFeng．zHANGJing．MedicalDepartment，AffiliatedHospitalofHebeiEngineeringUnivemity，Hebei，Handan056002，China【Abstract】0bjectiveToo

4、bservethetherapeuticeffectofCXCchemokinereceptor-4(cxcR4)．targetedtherapyonovariancancer．MethodsDMBAcottonthreadwasembeddedintotheOVariesofWisterratstoinduceovariancancermode1．Then60ratmodelswithovariancancerwererandomlydividedintoexperimentalgroupandcontrolgroupbymea

5、nsofrandomdigitstable．Theratsinexperimentalgroupweretreatedbygenetargetedtherapy，however，theratsincontrolgroupweretreatedbynegativetherapy．AftersiRNAsilencingovariancancerCXCR4gene，theexpressionofCXCR4protein，itseffectoncellproliferationofovariancancer，peritonealcavit

6、ymetastasisaftergenetargetedtherapywereobserved，andthesurvivalconditionofratsWasevaluated．ResultsAftertreatmenttheCXCR4levelsinascitesofexperimentalgroupweresignificantlydecreased(P<0．05)，however，theCXCR4levelsinascitesofcontrolgroupweresignificantlyincreased(P<0．05)．

7、TherewerenosignificantdifferencesintheCXCR4levelsinascitesbeforetreatmentbetweenexperimentalgroupandcontrolgroup(P>0．05)，however，thereweresignificantdifferencesintheCXCR4levelsinascitesaftertreatmentbetweenexperimentalgroupandcontrolgroup(P<0．05)．Ascomparedwiththatinc

8、ontrolgroup。theascitesextractionvolumeinexperimentalgroupwasobviouslydecreased(P<0．05)．ConclusionApplicationofgenetargetedth