还原型谷胱甘肽对脓毒症大鼠肺部超微结构及血中细胞因子水平的影响

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1、·282·中国危重病急救医学2OlO年5月第22卷第5期ChinCritCareMed，May2010，Vo1．22，No．5·论著·还原型谷胱甘肽对脓毒症大鼠肺部超微结构及血中细胞因子水平的影响廖秀玉林建东倪秀雄林辉【摘要】目的探讨还原型谷胱甘肽(GsH)对脓毒症大鼠急性肺损伤保护作用的机制。方法采用盲肠结扎穿孔术(CLP)复制SD大鼠脓毒症模型。按随机数字表法将大鼠分为假手术组、模型组、GSH组、左氧氟沙星(LEV)组；分别于术后3、6、12、24h各取7只大鼠心脏血检测血浆肿瘤坏死因子一n(TNF—a)、白细胞介素一6(IL～6)水平，

2、电镜下观察术后24h大鼠肺组织超微结构的改变。结果与假手术组[(132士9)~tg／L3比较，模型组术后6h血浆TNF—a水平[(227土28)~g／L3显著升高<0．01)；GSH治疗组[(144±28)~g／L]较模型组显著降低，且明显低于LEV组[(214士48)g／L，均尸

3、~g／L3较模型组显著降低，且明显低于LEV组((268．75士74．67)gg／L，均P

4、dpulmonaryultrastructureinratswithsepsisLIAOXiu—yu，LINJian-dong，NIXiu—xiong，LtNHui．IntensiveCareUnit，theFirstHospitalAffiliatedofFujianMedicalUniversity，Fuzhou350005，Fujian，ChinaCorrespondingauthor：，ⅣJian—dong，Email：linjd@tom．com[Abstract]ObjectiveToinvestigatetheprotection

5、mechanismofreducedglutathione(GSH)inacutelunginjuryinratswithsepsis．MethodsSepsisinSprague—Dawley(SD)ratswerereproducedbycecalligationandpuncture(CLP)．Theywererandomlydividedintofourgroups，sham-operatedgroup，modelgroup，GSHtreatmentgroupandlevofloxacin(LEV)treatmentgroup．Hea

6、rtbloodof7ratsinallgroupswascollectedat3，6，12，24hoursafteroperation．Theplasmalevelsoftumornecrosisfactor—(TNF—a)andinterleukin一6(IL一6)weremeasured．Thelunguhrastructurechangeswereobservedwithelectronmicroscopeat24hoursinallgroups．ResultsComparedwiththesham—operatedgroup，thep

7、lasmalevelofTNF—qincreasedmoreobviouslyat6hoursofthemodelgroup((227±28)ug／Lvs．(132±9)#g／L，P<0．01]．Comparedwiththemodelgroup，theplasmalevelofTNF一intheGSHtreatmentgroupdecreasedobviously[(144士28)gg／L]，anditwasobviouslylowerthanthatofLEVtreatmentgroup[(214士48)g／L，bothP<0．01]．N

8、oobviousdifferenceofplasmalevelofTNF—dwasfoundat3，12，24hoursamongallthegroups．Comp