曲霉临床和环境分离株基因型和药敏表型分析

《曲霉临床和环境分离株基因型和药敏表型分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、中华皮肤科杂志2010年9月第43卷第9期ChinJDermato]，September2010．Vo1．43．No．9·论著·曲霉临床和环境分离株基因型和药敏表型分析敖俊红杨燕妮郝飞【摘要】目的探讨临床和环境来源曲霉的基因分型情况以及不同基因型曲霉的药敏结果。方法采用随机扩增DNA多态性(RAPD)分型方法对45株烟曲霉、59株黄曲霉和30株黑曲霉进行基因分型，采用M38一A方案分析曲霉对抗真菌药物的敏感性。结果45株烟曲霉产生8种基因型，59株黄曲霉产生12种基因型，30株黑曲霉产生5种基因型。8种基因型烟曲霉对特比萘芬敏感性差异有统计学意义(=33．092

2、，P<0．OI)，而对其他抗真菌药物敏感性差异无统计学意义。5种基因型黑曲霉对两性霉素B敏感洼差异有统计学意义(=15．185，P<0．05)，而对其他抗真菌药物敏感性差异无统计学意义。结论不同型别的烟曲霉和黑曲霉对部分抗真菌药物的敏感性存在差异。【关键词】曲霉菌属；基因型；抗真菌药；微生物敏感性试验GenotypingandsusceptibilitytestingofAspergillusspeciesfromclinicalsettingsandenvironmentalsourcesAOJun—hong,YANGYan—ni，HAOFei．*Depart

3、mentofDermatology,SouthwestHospital，ThirdMilitaryMedicalUniversity，Chongqing430038，ChinaCorrespondingauthor：HAOEel，Email：haofei62@medmail．com．cn【Abstract】ObjectiveToinvestigatethegenotypeandantifungalsusceptibilityofAspergillusspeciesisolatedfromclinicalsettingsandenvironmentalsource

4、s．MethodsRandomamplificationofpolymorphicDNA(RAPD)analysiswasperformedtoprofilethegenotypesof48Aspergillus[umigatusstrains，59Aspergillusflavusstrainsand30Aspergillusnigerstrainsisolatedfromclinicalsettingsandenvironmentalsources．NCCLSM38一Aprotocolwascarriedoutforantifungalsusceptibil

5、itytestingoftheseAspergillusisolates．ResultsAsRAPDanalysisshowed，the48Aspergillusfumigatusstrainswereclassifiedinto8genotypes，59Aspergillustlavusstrainsinto12genotypes，and30Aspergillusnigerstrainsinto5genotypes．Asignificantdiferencewasobservedinthesusceptibilitytoterbinafineamongthe8

6、genotypesofAspergillusfumigatus(=33．092，P<0．01)aswellasinthattoamphotericinBamongthe5genotypesofAspergillusnr(=15．185，P<0．05)．NostatisticaldifferencewasfoundinthesusceptibilitytoamphotericinB，itraconazole，fluconazoleorflucyto—sineamongthe8genotypesofAspergillusfumigatusorinthattoterb

7、inafine，itraconazole，fluconazoleorflucy—tosineamongthe5genotypesofAspergillusniger．ConclusionThereissomediferenceinthesusceptibilitytosomeantifungalagentsbetweendiferentgenotypesofAspergillus[umigatusandAspergillusniger．【Keywords】Aspergillus；Genotype；Antifungalagents；Miembialsensitiv

8、itytests近年来，