乳酸菌的益生及免疫调节作用.ppt

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1、EvaluationofprobioticcharacteristicsofnewlyisolatedLactobacillusspp.:Immunemodulation乳酸杆菌的益生特性及免疫调节作用InternationalJournalofFoodMicrobiologyAvailableonline13May2011Keywords:LactobacilliProbioticcharacteristicsImmunemodulationInthecurrentstudy,theprobiot

2、icpotentialofapproximately350strainsoflacticacidbacteriaisolatedfromKoreaninfantfecesandKimchiwasinvestigated.Commonprobioticpropertiesofthebacterialstrains,suchasacidtolerance,biletoleranceandadhesiontohumanintestinalepithelialcells(HT-29cells),wereex

3、amined.Somestrainswerefoundtohaveimmunemodulatoryandantimicrobialproperties.Antagonisticactivityagainstapanelofpathogenicbacteriawasfoundtobestraindependent.Toevaluatetheimmunemodulatoryactivityofthestrains,lymphocyteinterferon(IFN)-γsecretionwasdeterm

4、inedinconjunctionwithcellproliferation.SomestrainsofLactobacillusgasseri,L.fermentumandL.plantarumexhibitedincreasedIFN-γlevelsandlymphocyteproliferation.Toevaluatetheeffectsoftheseimmunemodulatinglactobacillionhostlifespan,Caenorhabditiseleganswasused

5、asaninvivomodel.Nematodesthatweresuppliedheat-killedlactobacilliasafoodsourceexhibitedobviousdifferencesinlifespancomparedwiththosefedEscherichiacoliOP50.Themeanlifespan(determinedasmeanpercentsurvival)ofwormsfedL.plantarumCJLP133andL.fermentumLA12was1

6、3.89%and13.69%greater,respectively,thanthatofcontrolnematodesafter21days(P=0.036and0.043,respectively).Inaddition,someofsafetyprofiles,includinghemolytictype,gelatinhydrationanddegradationofurea,werefoundtobepositive.Thesenewlyidentifiedlactobacillihol

7、dpromiseforuseasprobioticagents,feedadditivesand/orinfoodapplications.Abstract研究发现，从韩国婴儿的粪便及泡菜中分离出的乳酸菌大约有350株菌株有益生潜力。益生菌的普遍益生特性主要包括：耐酸性，耐胆盐及对人肠上皮细胞的粘附性。还有一些菌株有免疫调节作用及抗菌作用。一些细菌对致病菌有一定的抵抗作用。为了评估这株菌的免疫调节作用，测定淋巴细胞干扰素（IFN）-γ的分泌与增值。一些发酵乳杆菌和植物乳杆菌会使干扰素（IFN）-γ分

8、泌增加及淋巴细胞的增值。一些安全指标，包括溶血性，明胶水化及尿素降解发现是阳性的。这些分离出来的菌株可以作为益生菌用于饲料及食物中。1Introduction乳酸菌作为益生菌可以有益于人类健康及阻止人类疾病。乳酸杆菌是一个有较好益生效果的菌属。大量的研究证实许多的乳酸杆菌都有益生特性。虽然在传统的食品中乳酸杆菌作为益生菌有很长的历史了，但它作为益生作用及用于食品加工之前一些指标还是需要测定的。益生菌能够抵抗胃酸和胆盐，并能够定植于人体的肠道中。已经证实乳酸菌能够抑制致