资源描述：
《冠状病毒Coronavirusl论文-1991 High level transient expression of the murine coronavirus haemagglutinin-esterase.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、JournalofGeneralVirology(1991),72,1309-1315.PrintedinGreatBritain1309Highleveltransientexpressionofthemurinecoronavirushaemagglutinin-esteraseMichaelPfleiderer,l~-EdwardRoutledge,l:~GeorgHerrler2andStuartG.Siddell1.1InstituteofVirology,UniversityofWftrzburg,VersbacherStrasse7,8700Wftrzburgand2Insti
2、tuteofVirology,UniversityofMarburg,Robert-Koch-Strasse17,3550Marburg,GermanyWehaveexpressedthemurinecoronavirushaemagglu-destroying(esterase)andreceptor-binding(haemad-tinin-esteraseproteininavacciniavirus/T7RNAsorption)activities.Theuseofthissystemwillgreatlypolymerasesystem.Thelevelsofexpressiono
3、bservedfacilitateanalysisofthestructure-functionrelation-aresignificantlyhigherthanthosefoundinvirus-shipsofthisprotein.infectedcells.Theexpressedproteinhasbothreceptor-Introductiontheseproteinswillbeassociatedwithchangesinviralpathogenicity.Thecoronavirusesareagroupofenveloped,positive-TheMHVHEgen
4、eencodesapolypeptideof439strandRNAvirusesassociatedwithdiseasesofeconom-aminoacidswhichisN-glycosylatedatnineorI0icimportanceinbothanimalsandhumans(Wegeetal.,positions(Shiehetal.,1989;Yokomorietal.,1989).In1982).Amongthemostintensivelystudiedmembersofthevirion,theHEproteiniscomposedofdisulphide-the
5、grouparethemurinecoronaviruses,collectivelylinkedhomodimersof120000Mr(Siddell,1982).Theknownasmurinehepatitisvirus(MHV).Theseviruseshomodimersareanchoredinthevirusmembraneandarecharacterizedbytheirabilitytocauseavarietyofformtheshortspikestructuresseeninnegativelystaineddifferentdiseasesdependingon
6、thevirusisolate,thehostpreparations(Sugiyama&Amano,1981).animalandtherouteofinfection.MHV-basedanimalAshasbeennotedpreviously,thepredictedaminomodelshavebeenestablishedforneurological,entericacidsequenceoftheMHVHEproteinshowsabout75%andhepaticdiseases(Lamontagneetal.,1989;Bartholdsimilaritywiththeb
7、ovinecoronavirus(BCV)HE&Smith,1989;Perlmanetal.,1990)anditishopedthatproteinand,moreremarkably,30%similaritywiththetheywillhelptoelucidateimportantaspectsofviralHEF1subunitoftheinfluenzaCvirusHEFproteinpath
