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1、GLIA2:318-329(1989)ExpressionofViralandMyelinGeneTranscriptsinaMurineCNSDemyelinatingDiseaseCausedbyaCoronavirusCRAIGA.JORDAN,'VICTORL.FRIEDRICH,JR.,'CATHERINEGODFRAIND,'CHRISTINEB.CARDELLECHIO,'KATHRYNV.HOLMES?ANDMONIQUEDUBOIS-DALCQ1'LaboratoryofViralandMo
2、lecularPathogenesis,NationalInstituteofNeurologicalDisordersandStroke,NationalInstitutesofHealth,Bethesda,Maryland20892;2DepartmentofPathology,UniformedServicesUniversityoftheHealthSciences,Bethesda,Maryland20814KEYWORDSMousehepatitisvirus,Remyelination,Ins
3、ituhybridization,Oligoden-drocyteABSTRACTC57BI/6NmicedevelopaCNSdemyelinatingdiseasewheninoculatedintracraniallyat4weeksofagewiththeA59strainofmousehepatitisvirus(MHV-A59).Inordertoexplorethevirus-hostinteractions,thehistologicalfeaturesofthedemyelinat-ingd
4、iseasewerecorrelatedwiththespatialandtemporaldistributionofviraltranscriptsandtheexpressionofoligodendrocyte-specificgenes(myelinbasicprotein,proteolipidprotein,myelin-associatedglycoprotein,and2',3'cyclicnucleotide3'-phosphohydrolase)inthespinalcordofdisea
5、sedmice.Threedistinctphasesinthediseasewereidentified.Inthefirstphase,1weekpostinfection(1WPI),virusreplicationwaswidespreadinbothgrayandwhitematterbutwaspreferentiallyoccurringinglialcells.Intheventralanddorsalrootzoneswhereviraltranscriptsweremostabundant
6、,allmyelingenetranscriptsweredecreasedbeforedemyelinationwasseen.Duringthesecondphaseofthedisease(2-3WPI),viraltranscriptsdecreasedinabundanceandbecamerestrictedtothewhitematter.Numerousdemyelinatinglesionswereobservedandwerecharacterizedbyinflammatorycells
7、,paucityofoligodendrocytes,andaprofounddecreaseofallmyelingenetranscripts.Inthethirdphaseofthedisease(4-6WPI)noviraltranscriptsweredetected,andremyelinationbegan.Inthelesionsandthetissuesurroundingthem,transcriptsofallmyelingenesincreasedtolevelsabovenormal
8、.Theincreasedexpressionofmyelingenetranscriptsoccurredinasynchronizedmannerandwithacellulardistributionreminiscentofthatseenindevelopmentalmyelination.Thesemoleculareventscorrelatedwithefficientremyeli
