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1、ArchivesofVirologyArchivesofVirology89,245--254(1986)©bySpringer-Verlagt986StructuralPolypeptidesoftheMurineCoronavirusDVIMByK.SUGIYAMA,1~.ISItIKAWA,andN.FUKIYtIAR2kDepartmentofBiology,FacultyofScience,tIirosakiUniversity,Hirosaki,JapanWith3FiguresAcceptedJuly16,1985Slllll
2、lllal)rThestructuralpolypeptidesofthenmrinecoronavirus])VIM(diarrhoeavirusofinfantmice)havebeenanalysedincomparisonwithotherstrainsMHV-2,MHV-3,MHV-4(JHM)andMHV-SbySDS-PAGE.Inthepresenceof2-mereaptoethanol,threemajorglyeopolypeptides,gp180,gp69,gp25(asagroupofsimilarspecies
3、)andonemajornon-glycosylatedpolypeptidep58weredetected.Thegp69isaDVIMspecificglyeopolypeptide,inwhichtheglycosidicmoietiesarelinkedtothecorepolypeptidethroughN-glycosidicbonds,andhencemaybecorrelatedwiththeshortprojectionsoftheviralenvelope.Ful'thergpl40,whichappearsinthea
4、bsenceofreducingagents,isapparentlyadimerofgp69heldtogetherbydisulfidelinkages.Thegp25family,ontheotherhand,consistsoffourpolypeptides,twoofwhichaxenotmetabolicallyinhibitedbytunicamyeinsuggestingthattheyareO-linkedglycopolypeptides.DVIMseemstobeserologicallycloselyrelated
5、totheMHV-SstrainasshownbyneutrMization.IntroductionCoronavirusesareagroupofenvelopedviruseswhichcontainlargesingle-strandedRNAgenomesofmessengerpolarity(t2,14,17,22).However,themurinecoronavirusescanbedistinguishedfromtheothercoronavirusesbythepresenceofO-linkedoligosaccha
6、rides(4,9)OurpreviousstudiesonthemorphologyandsomeofthebiologicalactivitiesofthemurineeoronavirusstrainDVIM(diarrhoeavirusofinfantmice)(18,19)haverevealedthepresenceofcharacteristicshortprojectionslocatedonthebaseof'club-shapedeoronaradiation'oftheDVIMvirion.Wewere246K.SUG
7、IYAMA,R.ISHIKAWA,andN.FUXU~IA~A:thereforeinterestedinstudyingthestructuralpolypeptideofthisvirusespeciallyincomparisonwiththoseofthewellcharacterizedmurinecorona-viruses.Inthisreportwedescribethecharacteristicmajorglycopolypeptidegp69,whichisexclusivelydetectedontheDVIMand
8、probablycorrespondstotheshortprojections.Wealsodescribetheeffectoftunicamycinonthesynthes
