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1、550BriefcommunicationsJVetDiagnInvest11:550±553(1999)Fatalcoronavirusinfectioninpuppiesfollowingcanineparvovirus2binfectionAnnamariaPratelli,MariaTempesta,FrancoP.Roperto,PaolaSagazio,LelandCarmichael,CanioBuonavogliaCaninecoronavirus(CCV)belongstotheCoronaviridaeindir
2、ect¯uorescentantibodytests(IFAT),andantigenicfamily,whichincludesenvelopedandpleomorphicvirusescharacterizationofisolateswithmonoclonalantibodies.All60±220nmindiameter,helicalnucleocapsids,andpetal4pupsrecovereduneventfullyandappearednormalafter1(club-shaped)peplomeres
3、,whicharewidelyspacedontheweek.envelope,resemblingasolarcorona.1,12Fifteendaysaftertheinitialoutbreak,3ofthe4pups(60CCVwas®rstisolatedin1971frommilitarydogsinGer-daysold)againhadsignsofhemorrhagicgastroenteritis,butmanysufferingfromgastroenteritis.3Subsequently,additio
4、n-signsweremuchmoreseverethanthoseinthe®rstepisode.alcaseshavebeenreportedthatwereusuallymildandself-Twopupsdiedwithin3daysaftertheonsetofclinicalsigns,limiting,unlesscomplicatedbycanineparvovirus(CPV)in-andthethirdpuprecovered.fection.2,7,8,10,14Coronaviraldiseaseindo
5、gsisextremelyvar-Grosspathologicexaminationofthe2deadpupsrevealediable.Althoughadultdogsgenerallyhavenoormildclinicalseverenecrotizinghemorrhagicenteritisinvolvingtheentiresignsandusuallyrecoverafterabriefperiodofillness,pupssmallintestine,andemphysematousfociwerescatt
6、eredoverwithsecondarybacterialinfections,parasites,orothervirus-thehyperemicandedematouslungs.Examinationforpara-esmaysuffersevere,evenfatal,disease.1,8,10Infecteddogssiteswasnegative,andbacteriologicculturesdidnotrevealshedCCVinthefecesfor6±9days,butsheddingcanbeanysi
7、gni®cantbacterialpathogens.prolongedinsomeanimals.1,9Themostlikelymodeoftrans-HAtestswereperformedonchloroform-treatedfecalmissionisbythefecal±oralroute.samples,using1%swineerythrocytesat4C,aspreviouslyThemaintargetofCCVisthesmallintestinalepithelium,described.5HAtests
8、alsowereperformedonthesupernatantwherealyticinfectionresultsindesquamationandshort-¯uidsofA-72cellculturesthathadbeen
