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1、[FrontiersInBioscience,Landmark,23,27-35,January1,2018]LeadersequencesofcoronavirusarealteredduringinfectionLiLiu1,WeiChen21DepartmentofMicrobiology,InstituteofBasicMedicalSciences,ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,Beijing10
2、0005,China,2BeijingInstituteofBiotechnology,No.20DongdajieStreet,FengtaiDistrict,Beijing100071,ChinaTABLEOFCONTENTS1.Abstract2.Introduction3.Materialsandmethods3.1.Cellculture,SARS-CoVstrainandviralinfection3.2.TheleadersequenceinformationofSARS-CoVa
3、ndMHV3.3.Primerdesignsandsequenceinformation3.4.RNAisolationandRT-PCRanalysis4.Results4.1.IdentificationofanovelsgmRNAfromHKU-39849strainofSARS-CoV4.2.AnalysisoftheleaderandTRSsequencesofsgmRNAsderivedfrombothSARS-CoVandMHV4.3.Thedifferentpatternsofl
4、eaderalterationsinMHVversusSARS-CoVgenome5.Discussion6.Acknowledgments7.References1.ABSTRACTThelifecycleofSevereAcuteRespiratorytheoutbreakofthefatalatypicalpneumoniaworldwideSyndromeCoronavirus(SARS-CoV)involvesauniquein2002–2003(1–3).Phylogenetican
5、alysisinitiallyprocesscalleddiscontinuoustranscriptionbywhichashowedthatSARS-CoVdidnotbelongtoanygroupsetof3’coterminalsubgenomicmRNAs(sgmRNA)ofknowncoronaviruses,butcouldbeclassifiedaswithidentical5’leadersequencescanbegenerated.group2bvirusbasedont
6、heconservationoftheThecurrentstudydemonstratesthatthereplicationcarboxylterminaldomainofspikeprotein(4).DueintermediatesofminusstrandofsubgenomicRNAtotherecentexpansionofnovelcoronaviruses,(sgRNA)canbereadilyrecoveredfromSARS-CoVtheviruseshavebeencla
7、ssifiedinto4generainfectedcells.AnovelsgmRNA(M-1)wasidentifiedas(alphacoronavirus,betacoronavirus,detacoronavirusashortversionofmembrane(M)gene.Transcriptionalandgammacoronavirus)basedonphylogeneticregulatorysequences(TRS)ofSARS-CoVandMouseanalysisan
8、dtheconservedsequencesofthenonHepatitisVirus(MHV)sgmRNAscontainaspeciesstructuralproteins,whileSARS-CoVwasre-groupedspecificcoreelement(CE).Thesizesofleaderasbetacoronavirus(5).sequencesinMHVsvarynotonlyindifferentviralstrainsbutalsoamongdifferentgen
