Environmental Microbiology 3.ppt

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1、welcome陈超宇严冬勤王巧燕鲍铮铮钱薇胡燕燕王小燕赵新景MicroorganismsOrganicPollutantsPollutantsPollutantsChemicpollutantsPhysicalpollutantsBiologicpollutantsOrganicpollutantsInorganicpollutantsPollutantsMicroorganismsartificialinartificialOrganicpollutantsHavethesuperpowertodestroy…Why???Potenti

2、alofthemicroorganismsDiversityinspeciesLargenumberDiversityinmetabolizingDistributingabroadThebasiccharacteristicsSotheycandealwiththegreatnumberofthevariousinartificialpollutantssuccessfullyPotentialofthemicroorganismsAbigfamilyPotentialofthemicroorganismsPotentialofthem

3、icroorganismsTotheartificialpollutants?artificialpollutants☆inlargernumber☆morekinds☆increaseatagreatspeedbothinnumberandkind☆newtotheworld(microorganisms)HardertodegradeMicroorganisms?PotentialofthemicroorganismsMorecharacteristics◎resistancetoenvironmentalstresses◎small

4、◎reproduceatafastspeedandindifferentways◎simplestructure◎easytoaberrance(变异)HavethePotentialtodealwiththeartificialpollutantsPotentialofthemicroorganismsPotentialofthemicroorganismsPotentialofthemicroorganismsIrondepositinanunidentifiedbacteriumstainedwithphenolcottonblue

5、.IsolatedfrombasinofautilityplantcoolingtowerPotentialofthemicroorganismsWhatwillthemicroorganismsdo?mutation(formnewkinds)inducement(formnewenzymesystem)Humanneedandcouldenhancetheirability(uwillclater…)constitutionofengineeringbacteriaTwomethods:1)Plasmid-assistedmolecu

6、larbreeding2)GeneticengineeringbreedingWhat???PlasmidPlasmidsaresmalldouble-strandedDNAmolecules.usuallycircular,thatcanexistindependentlyofhostchromosomesandarepresentinmanybacteria.PlasmidscantransfercopiesofthemselvestootherbacteriaduringconjugationPlasmidconjugativepl

7、asmidtransferintegrativeplasmidtransferrecombinantplasmidtransferTwomethodsPlasmid-assistedmolecularbreedingGeneticengineeringbreedingtransferofplasmidsartificialinartificialHybridizationofplasmidGeneticengineeringbreedingGeneisolationDNArecombineTransferbycarrierCopyande

8、xpressionFiltrationandbreedingGeneticengineeringbreedingBiodegradabilityoforganicpollutantsBiod