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1、麝珠明目滴眼液质量控制方法MingmueyedropsqualitycontrolmethodUpdateDate:2009-07-29Click:PanXin*JiLianfang,CengMinDeterminationofborneolMingmueyedropsintheabstractbygaschromatography.Thecontentofberberinehydrochloridewasdeterminedbythin-layerchromatography,withsatisfactory
2、results.Keywordsgaschromatography;thinlayerscanningmethod;Borneol;berberinehydrochloride;Mingmueyedropsconsistsofmusk,pearls,borneol,musk,Coptischinensisandotherherbs,includingpearlasmonarchdrug,borneol,Coptisasadjuvantdrug・Inordertocontrolthequalityofthispr
3、oduct,weusedgaschromatographytodeterminethecontentofberberinehydrochloride,andsatisfactoryresultswereobtained・1instrumentsandmaterialsPekin-Elemer8700gaschromatograph,SHIMADZUCS-930TLCseannerSHIMADZUDR-2processor,quantitativecapillary(Japan),borneol,berberin
4、ehydrochloride(ChinacontrolofpharmaceuticalandBiologicalProductsInstitute),silicaG(Qingdaooceanchemicalplant),Mingmueyedrops(LuJingmingeyewaterproducts)・2methodsandresults2.1gaschromatographyconditions:thecoatingconcentrationof9%0V-17isfixedliquid,columntemp
5、erature150degrees,detectorFID,thepeakseparationofborneolpeakandinternalstandardismorethan2.2,2thinlayerchromatographyconditions:adsorbent:silicagelG-CMC-Na;expansionagent:n-butanol-glacialaceticacid-water(7,1,3,2),reflectionmethod,sawtoothscanning,lambda二440
6、nm,speedoflightslit1.2,mm*1.2mm,linearparameterSX二二.1experimentalresults3.1determinationofborneolcontentPreparationof3.1.1internalstandardsolution:takefourteenalkane,dissolvewithethylacetate,andmakeitevery1mLcontaining10mgsolution,shakeitwel1.3.1.2standardso
7、lutionpreparation:takethestandardborneol8.50mgto5mL,thenadd1mLinternalstandardsolution,dilutewithaceticacidtothescale,shakeitwel1.3.1.3forsamplepreparation:Mingmueyedrops0.3gPrecisionsaid,addingether5mL,soakingovernight,centrifugation,thesupernatantandresidu
8、eplus5mL2hethylether,soaking,centrifugal,andsupernatant,quicklyswungtheresiduewithethylether,ethylacetatesolutionto5mLcapacitythebottle,add1mLinternalstandardsolution,constantvolume,i・e.・3.1.4pr
