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1、0°C冷藏下三文鱼片菌相变化规律及特定腐败菌的分离鉴定现代食品科技ModernFoodScienceandTechnology2015,Vol.31,No.40°C冷藏下三文鱼片菌相变化规律及特定腐败菌的分离鉴定李婷婷，丁婷，邹朝阳，周凯，仪淑敏，励建荣(1•大连民族学院生命科学学院，辽宁大连116600)(2.西南大学食品科学学院，重庆400715)(3•渤海大学食品科学研究院，辽宁省食品安全重点实验室，辽宁锦州)摘要：以冰鲜的三文鱼片为研究对象，利用形态学观察和16SrDNA序列分析法探究其在0°C并结合VITEK2微生物自动鉴定系统
2、对英特定腐败菌进行分离和鉴定。结果表明：0°C主要为葡萄球菌属(Staphylococcussp.)>假单胞菌属(Pseudomonassp.)>片球菌属(Pediococcussp.)、(菌属(Aeromonassp.)、肉食杆菌属(Carnobacteriumsp.)及部分鞘氨醇单胞菌属(渐单一，葡萄球菌属、片球菌属、而假单胞菌属呈上升趋势且到贮存后期数量占绝对优势。腐败菌的PCR软件构建系统发育树分析及微生物自动鉴定仪共同鉴定，确定荧光假单胞菌(关键词：三文鱼片；腐败菌；16SrDNA；发育树；荧光假单胞菌文章篇号：1673-907
3、8(2015)4-36-411,233332,3LITing-tingl,2,DINGTing33,ZHOUKai3?YlShu-min3,LIJiarvrong2,3ty,FoodSafetyKeyLabofnzhou121013,China)Abstract:Chilledfreshsalmonfilletswereusedforthestudy;thedifferentialprevaleneeofspoilagebacteriaduringstorageat0°Cwasanalyzedbymorphologicalobservat
4、ionsand16SrDNAsequeneeanalysis.Inaddition,thespecificspoilagebacteriawereseparatedandidentifiedusingtheVITEK2automatedmicrobialidentificationsystem.Themajorspoilagebacterialspeciesidentifiedinsalmonfilletsstoredat0°Cduringthestartofstorage(day0)wereStaphylococcussp.,Pseud
5、omonassp.zPediococcussp.,Micrococcussp.zAeromonassp.,Carnobacteriumsp.zandSphingomonassp.Thephaseofspoilagebacteriagraduallybecameunitarywiththeincreaseinstoragetime;asignificantdecreasewasobservedinthenumbersofStaphylococcussp.,Pediococcussp.,andMicrococcussp.,whereasthe
6、Aeromonassp.andCarnobacteriumsp;ontheotherhand,therewasagradualincreaseintheproportionofPseudomonassp.zleadingtoitspredominanceduringtheendofthestoragetime.Thesequences,amplifiedbypolymerasechainreaction(PCR),werecomparedagainstthesequencespresentintheNationalCenterforBio
7、technologyInformation(NCBI)database;thephylogenetictreeconstructedusingtheMEGA5.05softwarewasusedforanalysis,andtheautomatedmicrobialidentificationsystemwasutilizedforallmeasurements.TheseresultsidentifiedPseudomonasfluorescenstobethedominantspoilagebacteriuminrefrigerate
8、dsalmonfillets.Keywords:salmonfillets;spoilagebacteria;16SrDNA;phylogenetictree;Pseudomonasfluor
