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1、※生物工程食品科学2008,Vol.29,No.05327淡水养殖罗非鱼中病原菌和腐败菌的分离与鉴定初探刘寿春1，周康1，钟赛意1，李平兰1，马长伟1,*，彭朝辉2(1.中国农业大学食品科学与营养工程学院，北京100083；2.北京北水食品工业有限公司，北京101108)摘要：本实验分析了刚捕获淡水养殖罗非鱼的体表黏液、腮、肠道、腐败肉和养殖池水的细菌菌相，结果表明，以上样品的菌落总数分别为1.0×103CFU/cm2、2.2×103CFU/g、1.4×105CFU/g、5.4×107CFU/g和2.6×104CFU/ml；共分离出353株菌，以革兰氏阴性菌为主。池水和新鲜鱼
2、体的优势菌为芽孢杆菌、肠杆菌科、弧菌科，其主导地位随样品不同而有所差异，还有少量假单胞菌属、肠球菌属、葡萄球菌属等。腐败肉中的菌相较单一，优势菌为洋葱假单胞菌、嗜水气单胞菌和短芽孢杆菌，迟钝爱德华菌和克吕沃尔菌属少量。同时参照GB和SN标准对刚捕获罗非鱼的体表黏液、腮、肠道中病原菌进行分析，结果表明，在鱼体中检出大肠埃希氏菌、粪链球菌和铜绿假单胞菌，未检出单核增生李斯特氏菌、沙门氏菌、金黄色葡萄球菌、副溶血弧菌和溶血性链球菌等病原菌。关键词：罗非鱼；菌相；病原菌；腐败菌IsolationandIdentificationofPathogensandSpoilageBacter
3、iaonCulturedHybridTilapiaLIUShou-chun1，ZHOUKang1，ZHONGSai-yi1，LIPing-lan1，MAChang-wei1,*，PENGZhao-hui2(1.CollegeofFoodScienceandNutritionalEngineering,ChinaAgriculturalUniversity,Beijing100083,China；2.BeijingBeishuiFoodIndustryCo.Ltd.,Beijing101108,China)Abstract：Thebacterialfloraoccurringi
4、npondwater,surfacemucus,gills,intestineandputridmeatofhealthytilapiaculturedinfreshwater,wereestimatedbothquantitativelyandqualitatively.Theresultsshowedthattotalviablecountofbacteriainabovesamplesrespectivelywere2.6×104CFU/ml,1.0×103CFU/cm2,2.2×103CFU/g,1.4×105CFU/gand5.4×107CFU/g.Total353
5、isolatesweredominatedbyGram-negativebacteria.Bacillussp.,Enterobacteriaceaesp.andVibrionaceaesp.werepredominantinpondwaterandfreshfish,withasmallnumberofPseudomonassp,Enterococcussp.,Staphylococcussp.andsoon;butthedominantpositionwerechangedwithdifferentsamples.Thebacterialfloraweresimplein
6、putridmeat,Pseudomonascepacie,AeromonashydrophilaandBacillusbrevisweredominantspoilageorganism,withasmallnumberofEdwardsiellatardaandKluyveraspp.Inaddition,GBandSNstandardmethodswereusedindetectingthepathogensonsurfacemucus,gillsandintestineofnewly-harvestedtilapia,Escherichiacoli,Pseudomon
7、asaeruginosaandStreptococcusfaecalisweredetected,butListeriamonocytogenes,Salmonellasp.,Staphylococcusaureus,Vibrioparahaemolyticusandhemolyticstreptococcuswerenotdetected.Keywords：tilapia；bacterialflora；pathogens；spoilagebacteria中图分类号：S965.125文献标识码：A文章编
