人胎盘组织源造血干-祖细胞初步探究

《人胎盘组织源造血干-祖细胞初步探究》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、人胎盘组织源造血干/祖细胞初步探究作者：刘玉峰，张永卓，张传新，王叼【摘要】为了探讨足月胎盘组织源单个核细胞(humanmatureplacentatissue-derivedmononuclearcells,hPTMNC)中CD34+细胞的体外增殖、分化能力，寻找新的造血干/祖细胞来源供临床应用，分别采用流式细胞术检测、造血干/祖细胞集落培养和体外无细胞因子长期培养的方法，测定胎盘组织源和脐血中的CD34+细胞及其亚群和集落形成单位的数量。结果表明：胎盘组织源CD34+细胞(2.74±0.61%)及其亚

2、群CD34+/CD38-细胞(2.46±0.42%)、造血干/祖细胞集落CFU-GM(186.90±24.52)和BFU-E(101.40±13.35)水平都较脐血CD34+细胞(1.73±032%)、CD34+/CD38-细胞(0.80±0.25%)、CFU-GM(136・90±25・15)、BFU-E(49.20土&13)高；前者在体外无细胞因子培养条件下存活时间长，且细胞数量增加约2倍。结论：胎盘是胎儿期的造血器官，胎盘细胞成分更幼稚，是一种造血干/祖细胞移植的新的种子细胞。【关键词】胎盘组织Pre

3、liminaryStudyonHumanMaturePlacentaTissue-derivedHematopoieticStem/ProgenitorCellsAbstractClinicaltransplantationhasindicatedthatcordblood(CB)canbeusedinthehematopoieticreconstitutioninthechildren,butnotwellusedintheaduItpatientsbecauseofthelowcellamount.T

4、hepresentstudyaimedtoexplorethecapabilityofproliferationanddifferentiationofthehematopoieticstem/progenitorcellsderivedfromhumanmatureplacentatissue(PT)invitro,andtofindanewsourceofhematopoietic/progenitorcellsforclinicaltransplantation.CD34+cellsinhumanm

5、atureplacentatissuewereisolatedandcharacterizedbyusingenzyme-digestionmethodandflowcytometry.Alongculturesystemwithoutcytokineswasestablishedwithhumanmatureplacentatissue-derivedmononucleatedcellsandcordbloodmononuclearcells.Thenumberofnucleatedcellswaswe

6、eklycountedinculturefor14weeks・ThenumberofCFCwascountedinculturefor2weeks・TheresuItsshowedthattheCFCyields(CFU-GM,186.90±24.52；BFU-E,101.40±13.35)andthepercentageofCD34+cells(2.74±0.61%)andCD34+/CD38-cells(2.46土0.42%)inplacentatissue(PT)werehigherthanCFC(

7、CFU-GM,136.90±25.15；BFU-E,49.20±8.13),CD34+cells(1.73±0.32%)andCD34+/CD38-cells(0.80±0.25%)incordblood(CB).TheMNCsfromPThaveshownmoresurvivalabilitythanthecellsfromCBinthelong-1ermcellculturecondition；andthecellsfromPTincreasedby2times.Itisconcludedthatth

8、eplacentamaybeanotherhematopoieticorganinontogeny.Thecellsfromplacentaweremorejuvenile,andmaybefavorablesourceforclinicalstemcelltransplantation.Keywordsplacentatissue；cordblood；CD34+cell；hematopoieticstemcelltransp