早期声门型喉癌中血管、淋巴管生成特点及临床意义

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1、StudyonthecharacteristicsandclinicalsignificanceofAngiogenesisandlymphangiogenesisineariy-stagelaryngealcarcinomaAbstractObjective:Inrecentyears,manystudieshaveshownthattumorangiogenesisandlymphangiogenesisplayaimportantroleinthelocalanddistantmetastaticofdisease.Althoughmostearlyglotticcance

2、rafterconventionalsurgeryandradiationtherapycanachieveagoodprognosis,westillobservedasmallparthasapoorprognosisincasesoflocalrecurrence,organpreservationandsurvival•ThepurposeofourstudywastouseimmunohistochemicalexpressionofCD31andD2-40labeledtoinvestigatetheearlyglotticcancerangiogenicandlym

3、phangiogeniccharacteristicsandtheirpossibleprognosticimplicationsinpreviouslyuntreatedT1-T2glotticsquamouscellcarcinoma・Methods:PatientscomefromHunanProvincialPeoplefsHospitalwithpreviouslyuntreatedeariy-stagelaryngealcancerunderwentasurgicalresectionintheperiodbetween2007andDecember2011.13ca

4、seswithpooroutcomewereidentifiedandcomparedwithaselectedsampleof14patients.10casesinwhichspecimensand12casesofnormalmucosaspecimenswerecompared.Patientswerefollowedupuntildeathorforatleast24monthsafterfirsttreatment.Fromparaffin-embeddedtissuesectionslaryngealthreeconsecutiveslicethickness,pe

5、rformedimmunohistochemicalanalysis.D2・40andCD31monoclonalantibodiesareusedtomarkthebloodvesselsandlymphaticvessels.AntibodiesforD2・40andKi-67thatareusedtomarkthelymphaticendothelialcellproliferation,useImageProPlus6.0softwaretotesttheimmunohistochemicalimage.Amorphometricmeasurementwasusedfor

6、theanalysisofangiogenesiswhereaslymphangiogenesiswasstudiedwithasemiquantitativetechnique.WereanalyzedusingtheMann-Whitneyandchi-squarcmethodmethod.Results:Anincreasedtumorangiogenesiscorrelatedwithlocalrecurrence(P=0.011,P<0.05),death(P=0.034,P=0.043,P<0.05);peritumorallymphaticandlocalrecur

7、rencewassignificantlyassociated(P=0.004,P<0.05);diflerentinlaryngealcarcinomaproliferationoflymphaticendothelialcellsindifferentregions;peritumorallymphaticendothelialcellproliferationhighestactivitywassignificantlyhigherthanintumor(P<0.05);p