早期声门型喉癌中血管、淋巴管生成特点及临床意义.doc

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1、StudyonthecharacteristicsandclinicalsignificanceofAngiogenesisandlymphangiogenesisineariy-st^elaryngealcarcinomaAbstractObjective:Inrecentyears,manystudieshaveshownthattumorangiogenesisandlymphangiogenesisplayaimportantroleinthelocalanddistantmetastaticofdisease・Althoughmo

2、stearlyglotticcancerafterconventionalsurgeryandradiationtherapycanachieveagoodprognosis,westillobservedasmallparthasapoorprognosisincasesoflocalrecurrence,organpreservationandsurvival•ThepurposeofourstudywastouseimmunohistochemicalexpressionofCD31andD2-40labeledtoinvestiga

3、tetheearlyglotticcancerangiogenicandlymphangiogeniccharacteristicsandtheirpossibleprognosticimplicationsinpreviouslyuntreatedT1-T2glotticsquamouscellcarcinoma・Methods:PatientscomefromHunanProvincialPeople!sHospitalwithpreviouslyuntreatedearly-stagelaryngealcancerunderwenta

4、surgicalresectionintheperiodbetween2007andDecember2011」3caseswithpooroutcomewereidentifiedandcomparedwithaselectedsampleof14patients.10casesinwhichspecimensand12casesofnormalmucosaspecimenswerecompared.Patientswerefollowedupuntildeathorforatleast24monthsafterfirsttreatment

5、.Fromparaffin-embeddedtissuesectionslaryngealthreeconsecutiveslicethickness,performedimmunohistochemicalanalysis.D2・40andCD31monoclonalantibodiesareusedtomarkthebloodvesselsandlymphaticvessels.AntibodiesforD2-40andKi-67thatareusedtomarkthelymphaticendothelialcellproliferat

6、ion,useImageProPlus6.0softwaretotesttheimmunohistochemicalimage.Amorphometricmeasurementwasusedfortheanalysisofangiogenesiswhereaslymphangiogenesiswasstudiedwithasemiquantitativetechnique・WereanalyzedusingtheMann-Whitneyandchi-squarcmethodmethod.Results:Anincreasedtumorang

7、iogenesiscorrelatedwithlocalrecurrence(P=0.011,P<0.05),death(P=0.034,P=0.043,P<0.05);peritumorallymphaticandlocalrecurrencewassignificantlyassociated(P=0.004,P<0.05);differentinlaryngealcarcinomaproliferationoflymphaticendothelialcellsindifferentregions;peritumorallymphati

8、cendothelialcellproliferationhighestactivitywassignificantlyhigherthanintumor(P<0.05);per