地塞米松诱导CEM细胞凋亡过程中Survivin和Caspase-3基因表达的研究

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1、【播要】目的：探讨地皇米松(dexameihasone

2、Westernblot检测Survivin和Caspase-3蛋白表达$RT-PCR检测Survivin基因表达。结果：1)DEX以时间、刑量依赖方式抑制CEM细胞增殖。0・】〜】0pmol/LDEX于48h开始明显抑制CEM细胞的生长.24.48.72h抑制细胞增殖50%的药物浓度分别为9.8.0.9和0.

3、4Mmol/L02〉随DEX刑量增大亚二倍体细胞百分敛逐渐增加.5pmol/LDEX处理12〜48h.亚二倍体细胞从14.9%升至46.2%,3)5pmol/LDEX处理12〜72htSurvivin蛋白从54.6%下调至9.7%tSurvivinmRNA从76.4%(6h)降至18・3%(72h)；4)DEX作用12h之内只能检测到非激活状态的procaspase-3■处理24h后出现17X10’活性亚恳•克至72h.结论:DEX诜导CEM细胞凋亡时下调Survivin屋因表达•活化Caspase3•中华肿■防治^^>2006«13(19)<1464-1467【

4、中图分类号】：R733.71地塞米松诱导CEM细胞凋亡过程中Survivin和Caspase-3基因表达的研究何莉，刘云鹏，侯科佐，朱志图，李丹，张敬东中国医科大学附属第一医院(儿科：何莉；肿瘤内科：刘云鹏，侯科佐,朱志图，李丹，张敬东)，辽宁沈阳110001ResearchongeneexpressionsofSurvivinandCaspase-3indexamethasone-inducedapoptosisinCEMcellsHELi9LIUYunpwHOUKe-zuo«ZHUZhi-tu9LIDan9ZHANGJing^dongNo.1Hospitalo

5、fChinaMedicalUniversity9Shenyang1100019P.R.China[ABSTRACTOBJECTIVE：Toinvestigategeneexpressionsofsurvivinandcaspase-3indexamethasoneinducedapoptosisinCEMcells.METHODS：Cellviabilitywasdeterminedbythetrypanbluedyeexclusionbyusingahcmocytometer.Apoptosiswasassessedbymorphologyandflowcyto

6、metry.Proteinexpressionofsurvivinandcaspase-3wasanalyzedbyWesternblot.SurvivinmRNAexpressionwasmeasurededbyRTPCR・RESULTS；Proliferationofdexamethasone-inducedCEMcellswasinhibitedinaconcentration-andlimedependentmanner・TheICsoat24h・48hand72hwere9.8pmol/Lt0.9pmol/Land0.4pmol/I-trespectiv

7、ely.Apoptosisincreasedfrom14.9%to46.2%aftertreatmentwith5pmol/I“dexamethasonefrom12hto48h.Comparedwiththecontrol,proteinexpressionofsurvivinwasdownregulatedfrom54.6%(12h)to9.7%(72h).ComparedwiththecontrolssurvivinmRNAex*pressiondecreasedfrom76.4%to18.3%aftertreatmentwith5pmol/Ldexamet

8、hasonefrom6to72h・Thecleavedactivesubunitsofcaspase・3wereobservedfollowingdexamethasonetreatmentfrom24to72h.CONCLUSIONS:lkwxamcthasone-inducedapoptosisinCEMcellsisassociatedwithdownregulationofsurvivinexpression»activationofcaspase3.ChinJCancerPrevTreat.2006f13(19)：H641167【关律词】地宝米松/药理学

9、『白血病/