[9]supporting information英文学习材料

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1、SupportingInformationMeieretal.10.1073/pnas.1014853108SITextidentifiedasthesameobjectinthenextframe.InfluorescenceFiniteElementComputation(FEC).Forallnumericalcalculations,atimages,eachbrightnessclusterrepresentsasingleDictyosteliumleast70,000gridpointsin2-Dand200,000gridpo

2、intsin3-Darediscoideumcell,andyieldsitscenterofmassposition,area,andused;allcalculationsareperformedwithCOMSOLMultiphysicsperimeterasafunctionofexperimenttime.3.5(Mathworks).ThehydrodynamicvelocityfieldiscomputedusingtheStokesequation,theconcentrationfieldusingthecon-Fluor

3、escenceDistributionMoment(FDM)Analysis.Toextractthevection-diffusionequation.No-slipboundaryconditionsareusedFDM,theintegratedproductofthefluorescenceintensityFIatthesidewalls.Aconstantinflowspeedof300μm∕sinthesideofeachpixelanditsdisplacementfromthecellcenterofmassflowsan

4、d250μm∕sinthecentralflowisassumedinthecalcula-displottedasa2-Dvector.ThehomemadeImageJpluginCel-tions.Vanishingviscousstressisassumedasboundaryconditionfortheoutflow.AdiffusioncoefficientofD¼5×10−10m2∕s,nolEvaluator(seeabove)isusedtofindthiscenterofmass.Theconcentrationflu

5、xboundaryconditionsatthewalls,arelativeFDMbecomespositiveifitsvectorpointsparalleltothedirectionchemoattractantconcentrationof1attheouterinletsand0atofthegradientandnegativeiforientedintheoppositedirection.theinnerinlet,areemployed.TheoutflowofconcentrationisHence,theFDM(i

6、nanalogytoanelectricdipolarmoment)describedbyaconvectivefluxboundarycondition,whichallowsyieldsthecellpolarization,asidentifiedbyactinpolymerizationforspeciestransportbyconvectiononly.dynamicsinLim-GFPcells.Asthegradientdirectioninoursetupswitchesinadefinedaxis,itissuffici

7、enttousetheamplitudeofAnalysisofCellMigrationbytheCellEvaluator.Thesinglefluores-theresultingvector.TheFDMisthennormalizedsothatallcellscencecelltrackingpluginCellEvaluator(homemade)detectsexhibitcomparableFDMvalues.Highoverallcellfluorescencecellsasclustersofnbrightpixels,

8、aboveanintensitythresholdintensityIwillresultinlargeFDMvaluesandanincreaseinareaI0andincl