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《Mesenchymal Stem Cell-Derived Exosomes PromoteFracture Healing in a Mouse Model间充质干细胞来源的外泌体促进 小鼠骨折愈合的实验研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、Tissue-SpecificProgenitorandStemCellsTISSUE-SPECIFICPROGENITORANDSTEMCELLSMesenchymalStemCell-DerivedExosomesPromoteFractureHealinginaMouseModela,*a,b,*bccTAISUKEFURUTA,SHIGERUMIYAKI,HIROYUKIISHITOBI,TOSHIHIKOOGURA,YOSHIOKATO,a,bdbaNAOSUKEKAMEI,KENJIMIYADO,YUKIHITOHIGASHI,MITSUOOC
2、HIKeyWords.FracturehealingxMesenchymalstemcellsxExosomesxEndochondralossificationxCytokinexmicroRNAaDepartmentofOrthopaedicsABSTRACTSurgery,IntegratedHealthParacrinesignalingbybone-marrow-derivedmesenchymalstemcells(MSCs)playsamajorroleinSciences,Instituteoftissuerepair.Althoughth
3、eproductionofregulatorycytokinesbyMSCtransplantationisacriticalBiomedicalandHealthmodulatoroftissueregeneration,wefocusedonexosomes,whichareextracellularvesiclesthatcon-Science,Hiroshimatainproteinsandnucleicacids,asanoveladditionalmodulatorofcell-to-cellcommunicationandUniversity
4、,Hiroshima,Japan;tissueregeneration.Toaddressthis,weusedradiologicimaging,histologicalexamination,andbimmunohistochemicalanalysistoevaluatetheroleofexosomesisolatedfromMSC-conditionedme-DepartmentofRegenerative2/2dium(CM)inthehealingprocessinafemurfracturemodelofCD9mice,astraintha
5、tisknowntoMedicine,MedicalCenterfor2/2producereducedlevelsofexosomes.WefoundthattheboneunionrateinCD9micewassignif-TranslationalandClinicalicantlylowerthanwild-typemicebecauseoftheretardationofcallusformation.TheretardationofResearch,HiroshimafracturehealinginCD92/2micewasrescuedb
6、ytheinjectionofexosomes,butthiswasnotthecaseUniversityHospital,aftertheinjectionofexosomes-freeconditionedmedium(CM-Exo).Thelevelsofthebonerepair-Hiroshima,Japan;relatedcytokines,monocytechemotacticprotein-1(MCP-1),MCP-3,andstromalcell-derivedfactor-1cBiomedicalResearchinexosomesw
7、erelowcomparedwithlevelsinCMandCM-Exo,suggestingthatbonerepairmaybeInstitute,NationalInstituteofinpartmediatedbyotherexosomecomponents,suchasmicroRNAs.TheseresultssuggestthatAdvancedIndustrialScienceexosomesinCMfacilitatetheaccelerationoffracturehealing,andweconcludethatexosomesar
8、eandTechnology,Tsukuba,anovelfact
