Coeloglossum viride var. bracteatum extract attenuates dgalactose and NaNO2 induced memory impairment in mice

《Coeloglossum viride var. bracteatum extract attenuates dgalactose and NaNO2 induced memory impairment in mice》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、JournalofEthnopharmacology104(2006)250256Coeloglossumviridevar.bracteatumextractattenuatesd-galactoseandNaNO2inducedmemoryimpairmentinmiceDanZhang,GengtaoLiu,JiangongShi,JianjunZhang∗DepartmentofPharmacology,InstituteofMateriaMedica,ChineseAcademyofMedicalSciences&PekingUnionMedicalColleg

2、e,Beijing100050,ChinaReceived4May2005;receivedinrevisedform3September2005;accepted7September2005Availableonline10October2005AbstractTheaimistostudytheneuroprotectiveeffectsofCE,aspecialextractfromCoeloglossumviride(L.)Hartm.var.bracteatum(Willd.),onmemorydeficitsinsenescentmiceinducedbycon

3、secutiveinjectionofd-galactoseandNaNOfor60days.OraladministrationofCE(2.5mgkg−1,25mgkg−1)startedfrom47thdayoftheexperiment.Watermazetestwasusedtoevaluatethelearningandmemoryfunctionofmice.ThecontentsofMDAandGSHandtheactivitiesofSODandGSH-pxinbraintissueweredeterminedusingdifferentbiochemi

4、calmethods.Thealterationsinhippocampusmorphologywereassessed.ImmunoreactivecellsofTau-2andNT-3inthehippocampuswerecountedbyimmunohistochemicalstaining.Theresultsindicatedthatcombinedinjectionofd-galactoseandNaNO2inducedmemoryimpairmentofmice.Inaddition,SODandGSH-pxactivitiesandGSHleveldec

5、reasedwhileMDAlevelincreasedinmousebrain.Tau-2positiveneuronsincreasedwhileNT-3positiveneuronsdecreasedremarkablyinthehippocampus.AdministrationofCEfor14dayssignificantlyimprovedthecognitivedeficitsandthebiochemicalmarkersmentionedaboveandreducedthehistologicallesionsinmousebrain.Theseresul

6、tssuggestthatCEisworthtestingforfurtherpreclinicalstudyaimedforsenescenceanddementia.©2005ElsevierIrelandLtd.Allrightsreserved.Keywords:Galactose;NaNO2;Oxidativestress;Learningandmemory;Senescence1.IntroductiontoinvestigatethechemicalcomponentsofCoeloglossumviridevar.bracteatum(Huangetal.

7、,2004).ThroughspecificCoeloglossumviride(L.)Hartm.var.bracteatum(Willd.)isextractionandseparation,anactiveextractfromtherhizomeaplantofOrchidaceaefamilydistributedintheNorthwestofwasobtainedandnamedCE.TheextractiontechniquewasChina,suchasTibet,InnerMongol,ShanxiProvi