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1、RESEARCHARTICLEPlasmidDNAVaccineCo-ImmunisationModulatesCellularandHumoralImmuneResponsesInducedbyIntranasalInoculationinMiceDeborahF.L.King,PaulF.McKay,JamieF.S.Mann,C.BrynJones,RobinJ.Shattock*MucosalInfectionandImmunityGroup,DepartmentofInfectiousDisea
2、ses,DivisionofMedicine,ImperialCollege,London,UnitedKingdoma11111*r.shattock@imperial.ac.ukAbstractOPENACCESSBackgroundCitation:KingDFL,McKayPF,MannJFS,JonesAneffectiveHIVvaccinewilllikelyrequireinductionofbothmucosalandsystemiccellularCB,ShattockRJ(2015)
3、PlasmidDNAVaccineCo-andhumoralimmuneresponses.Weinvestigatedwhetherintramuscular(IM)deliveryofImmunisationModulatesCellularandHumoralelectroporatedplasmidDNAvaccineandsimultaneousproteinvaccinationsbyintranasalImmuneResponsesInducedbyIntranasalInoculation
4、inMice.PLoSONE10(11):e0141557.(IN)andIMroutescouldbecombinedtoinducemucosalandsystemiccellularandhumoraldoi:10.1371/journal.pone.0141557immuneresponsestoamodelHIV-1CN54gp140antigeninmice.Editor:ShixiaWang,UniversityofMassachusettsMedicalSchool,UNITEDSTATE
5、SResultsReceived:March25,2015Co-immunisationofDNAwithintranasalproteinsuccessfullyelicitedbothserumandvagi-nalIgGandIgAresponses,whereasDNAandIMproteinco-deliverydidnotinducesys-Accepted:October9,2015temicormucosalIgAresponses.CellularIFNγresponseswerepre
6、servedinco-Published:November6,2015immunisationprotocolscomparedtoprotein-onlyvaccinationgroups.TheadditionofDNACopyright:©2015Kingetal.ThisisanopenaccesstoINproteinvaccinationreducedthestrongTh2biasobservedwithINproteinvaccinationarticledistributedundert
7、hetermsoftheCreativealone.Luminexanalysisalsorevealedthatco-immunisationwithDNAandINproteinCommonsAttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanyinducedexpressionofcytokinesthatpromoteB-cellfunction,generationofTFHcellsand
8、medium,providedtheoriginalauthorandsourceareCCR5ligandsthatcanreduceHIVinfectivity.credited.DataAvailabilityStatement:AllrelevantdataareSignificanceavailableinthepaperanditsSupportingInformationfiles.Thesedatasugges
