2-Sulforaphane attenuates matrix metalloproteinase-9 expression following spinal cord injury in mice

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1、Availableonlineatwww.annclinlabsci.org354AnnalsofClinical&LaboratoryScience,vol.40,no.4,2010SulforaphaneAttenuatesMatrixMetalloproteinase-9ExpressionFollowingSpinalCordInjuryinMiceLeiMao,HanDongWang,XiaoLiangWang,LiangQiao,andHongXiaYinDepartmentofNeurosurgery,JinlingHospit

2、al,SchoolofMedicine,NanjingUniversity,Nanjing,JiangsuProvince,ChinaAbstract.Inflammationplaysanimportantroleinthepathogenesisofsecondarydamageafterspinalcordinjury(SCI).Thepresentstudyexploredtheeffectofsulforaphane(SFN),apotentanti-inflammatoryextractofcruciferousvegetable

3、s,ontheexpressionoftwoinflammatorymediators,metalloproteinase(MMP)-9andTNF-α,inamurinemodelofSCI.Murinespinalcordinjurywasinducedbytheapplicationofvascularclips(forceof10g)totheduraafterathree-levelT8-T10laminectomy.Thewet/dryweightratiowasusedtoreflectthepercentageofwaterc

4、ontentofimpairedspinalcordtissueat48hrafterSCI.ThemRNAlevelsofMMP-9weredeterminedusingthereverse-transcriptasepolymerasechainreaction(RT-PCR),andproteinlevelsofTNF-αandMMP-9weredetectedbyenzyme-linkedimmunosorbentassays(ELISA)at24hrafterSCI.Gelatinzymographywasusedtodetermi

5、neMMP-9activityofspinalcordtissueat24hrafterSCI.MicetreatedwithSFNat1hrafterSCIhadlowerexpressionandactivityofMMP-9comparedtomicewithSCI.ThedecreaseofMMP-9inmicetreatedwithSFNwasassociatedwithdecreasedlevelsofspinalcordwatercontentandTNF-α.Insummary,suforaphanedecreasesMMP-

6、9andTNF-αexpressionandvascularpermeabilitychangesfollowingspinalcordinjuryinmice.Keywords:spinalcordinjury,sulforaphane,matrixmetalloproteinase-9,TNF-αIntroductioninjuryandprotecttheneuralelementsthatinitiallysurvivethemechanicalinjury.Furthermore,evenaSpinalcordinjury(SCI)

7、isadevastatingpathologysmallgaininneuroprotectionmightinducethatmayleadtopermanentdisability.Itisgenerallyfunctionallyrelevantneurologicrecovery[2].acceptedthatSCIinitiatesacascadeofcellularandMMPsareafamilyofsolubleandcell-surfacemoleculareventsandthatacombinationofsecond-

8、boundzinc-dependentendopeptidasesthatmediatearyinjuryfactorsresultsinprogressivene