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1、CancerBiologyTechnologyinCancerResearch&TreatmentFascinOverexpressionPromotes2016,Vol.15(2)322–333ªTheAuthor(s)2015CholangiocarcinomaRBECellReprintsandpermission:sagepub.com/journalsPermissions.navDOI:10.1177/1533034615580696Proliferation,Migration,andInvasiontct.sagepub.com1,211HaiyingZhao
2、,MD,FuquanYang,MD,WenyanZhao,MM,11,2ChunjvZhang,MM,andJingangLiu,MMAbstractFascinisoverexpressedinvarioustumortissuesandiscloselyrelatedtotumormetastasisandinvasion.However,theroleoffascinincholangiocarcinomaRBEcellshasnotbeenclearlyreported.Thisstudyaimedtoestablishacholangiocarcinomacelll
3、inewithstableandhighexpressionoffascintoobservetheeffectoffascinoncellproliferation,migration,andinvasion.Afascinover-expressionvector,pcDNA3.1-Fascin,wasconstructedandtransfectedintothehumancholangiocarcinomaRBEcellline.Theresultsofreal-timepolymerasechainreaction,Westernblot,andimmunofluo
4、rescenceindicatedthatfascinwassteadilyandhighlyexpressedinRBEcells.Theresultsof3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromideandcolonyformationassayindicatedthatupregulatedfascinexpressioncouldenhancecholangiocarcinomacellproliferation.Theresultsofwoundhealingassayandtranswella
5、ssayindicatedthatfascincouldpromotecholangiocarcinomacellmigrationandinvasion,andafurtherstudyfoundthatthenuclearfactor-kBsignalingpathwaywasactivatedafterupregulationoffascin,whereasE-cadherinexpressioninthesecellswassignificantlydecreased.Additionally,E-cadherinexpressionwassignificantlyi
6、ncreasedafterinhibitingnuclearfactor-kBactivityusinginhibitororsmallinterferingRNA,andE-cadherinexpressionwasdecreasedbyfascinoverexpressionafternuclearfactor-kBinhibition,suggestingthatnuclearfactor-kBsignalingpathwaywasnotinvolvedintheregulationofE-cadherinbyfascin.Insummary,theresultsoft
7、hisstudydemonstratedthatfascineffectivelypromotedcholangiocarcinomaRBEcellproliferation,migration,andinvasion.Thisstudyprovidesevidenceforfascinasapotentialtargetinthetreatmentofcholangiocarcinoma.Keywordsfascin,cholangiocarcinoma,proliferation,migration
