MicroRNA regulation of myogenic satellite cell proliferation and differentiation

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1、MolCellBiochem(2016)412:181–195DOI10.1007/s11010-015-2625-6MicroRNAregulationofmyogenicsatellitecellproliferationanddifferentiation11RachelL.Harding•SandraG.VellemanReceived:23September2015/Accepted:8December2015/Publishedonline:29December2015ÓSpringerScience+Busine

2、ssMediaNewYork(outsidetheUSA)2015AbstractMyogenicsatellitecellsarestemcellsrespon-Introductionsibleformusclegrowthandregeneration.MicroRNAs(miRNAs)playsignificantrolesinregulatingnumerousDuringembryonicdevelopment,myogenicprogenitorcellscellularprocesses.Twogenesesse

3、ntialtosatellitecellcalledmyoblastsfusetoformmultinucleatedmyotubesfunctionaresyndecan-4andglypican-1.Todetermineifthatdifferentiateintoskeletalmusclemyofibers.WhilemiRNAsinfluencemyogenicsatellitecellfunction,onedifferentiatedmusclefibersarefullyformedatbirthormiRNApr

4、edictedtobindsyndecan-4(miR-128)andtwohatch[1],allpostnatalorposthatchmusclegrowthoccurspredictedtobindglypican-1(miR-24andmiR-16)werethroughhypertrophyofexistingmusclefibersandisinhibitedinvitrobytransfectionofinhibitorstargetingmediatedbysatellitecells[2].Myogenics

5、atellitecellseachmiRNA.InhibitionofthesemiRNAsdifferentiallywerefirstidentifiedbyMauro[3]betweenthebasementaffectedtheexpressionofsyndecan-4,glypican-1,andmembraneandsarcolemmaofmyofibers.DuringposthatchmyogenicregulatoryfactorsmyoDandmyogenin.Inhibi-skeletalmusclegrow

6、thandregeneration,satellitecellstionofmiR-16reducedproliferationofsatellitecellsatproliferateandfusewithexistingmyofibers,addingaddi-72h.InhibitionofmiR-128andmiR-24didnotaffecttionalnucleitothefiberandincreasingproteinsynthesisproliferation.InhibitionofmiRNAsreducedd

7、ifferentiationpotential[2,4].Satellitecellsareadditionallycapableofofsatellitecellsintomyotubesat48and72hexceptforfusingtooneanother,formingnewmyofibers[2].miR-16,whichonlyaffecteddifferentiationat72h.Inhi-Satellitecellsareaheterogeneouspopulationofmulti-bitionofallt

8、hreemiRNAsdecreasedmyotubewidthatpotentstemcells[5,6]thathavedifferentfunctional24hofdifferentiationandincreasedmyotubewidthatcharacterist