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1、ActinomycinDVM-26onratgliomacellproliferationinvivostudyoftheeffectsofAuthors:WUYong-Kang,Lun,Hua-WeiLiu,ShenLin[Abstract]ObjectiveTostudytheactinomycinD(ActinomycinD,ACTD),andteniposide(Teniposide,VM-26)onratgliomacellsinvivotherapeuticeffect.Methodsofwild-typeC6ratgliomacell
2、sinoculatedinmousebrainontherightcaudatenucleus(controlgroup10,no-loadgroupof10rats),andhasbeentheformationofC6ratbraingliomaandVMrespectivelyACTD-26anti-tumorinsituinjectionofsustained-releaseagenttumorarea(treatmentgroup=10).Observedinratsineachgroupingeneral,survival,tumorp
3、athologyandmagneticresonanceimaging(MRI)dynamicchange,usingPCNAcounts,TUNELassayoftumorcellproliferationactivityandapoptosis.ResultsInthecontrolgroupandno-loadaveragesurvivalperiodofrats19.3days,ACTDSection6,andVM-26group3ratsweresignificantlyprolongedsurvivaltime,survivalperi
4、odofmorethan200days;Inadditiontohumandeathduetopathologicalexaminationoftwothings,ACTDgroupdiedwithin4weeksaftertreatment2,VM-26Section5.MRIexaminationin16controlgroupsignificantlyinratbraintumorfoci,thetreatmentgroupintheratbraintumorfocidecreasedordisappearedaftertreatment,a
5、reconsistentwiththepathologicalexamination,andthetreatedratsreducedC6cellproliferation,alargenumberofcells,witherdeath,ACTDsignificantlyhigherthanVM-26.ConclusionACTDlocalapplicationinvivoisexpectedtobecomethepreferredtreatmentofgliomachemotherapy.[Keywords:]actinomycinD;Tenip
6、oside;nerveglioma;body;chemotherapy[Abstract]ObjectiveTostudythetherapeuticeffectofACTDandVM-26onratC6gliomainvivo.MethodsWildtypeC6cellswereimplantedstereotaxicallytothecaudatenucleusofrightcerebrumofWistarrats(controlandemptyloadinggroup,10ratsrespectively),andratswithcerebr
7、altumorfociweretreatedACTDandVM-26mediatedbydelayedreleaseretarder(treatedgroup,10ratsrespectively).Thegeneralmanifestation,survivaltime,MRIfeatures,histopathologicalchange,proliferationactivityandapoptosisofthegliomain16eachgroupofratswereobserved.ResultsThemeansurvivaltimeof
8、controlanimalswas19.3days.TwoACTDorVM-26treatedratswerekilled
