17. Overexpression of LncRNA AFAP1-AS1 predicts poor prognosis and promotes cells proliferation and invasion in gallbladder cancer.

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1、Biomedicine&Pharmacotherapy84(2016)1249–1255AvailableonlineatScienceDirectwww.sciencedirect.comOverexpressionofLncRNAAFAP1-AS1predictspoorprognosisandpromotescellsproliferationandinvasioningallbladdercancera,1b,1bbb,**,c,*FeiMa,Shou-HuaWang,QiangCai,Ming-DiZhang,YongYangJunDingaDepartmen

2、tofOncology,XinhuaHospital,ShanghaiJiaotongUniversitySchoolofMedicine,Shanghai,200092,ChinabDepartmentofGeneralSurgery,XinhuaHospital,ShanghaiJiaotongUniversitySchoolofMedicine,Shanghai200000,ChinacDepartmentofBiliaryandPancreaticSurgery,ShanghaiShuguangHospitalAffiliatedwithShanghaiUnive

3、rsityofT.C.M.,Shanghai,201203,ChinaARTICLEINFOABSTRACTArticlehistory:Background:Longnon-codingRNAactinfilament-associatedprotein1antisenseRNA1(AFAP1-AS1)hasReceived19August2016beenelucidatedtobeassociatedwithsomekindsofhumancancers.However,whetherlncRNAAFAP1-Receivedinrevisedform13October

4、2016AS1Acceptedimplicatesintumordevelopmentofgallbladdercancer(GBC)remainslargelyunknown.Thisstudy21October2016aimstoelucidatethetumorigenicroleandregulatoryfunctionoflncRNAAFAP1-AS1ingallbladdercancer.Keywords:Methods:GallbladderWeanalyzedlncRNAAFAP1-AS1expressionbyquantitativerealtimeP

5、CR(qRT-PCR)in40cancergallbladderlncRNAcancertissueandadjacentnormaltissues,survivalplotsweregeneratedbyKaplan-MeierAFAP1-AS1analysisEpithelial-mesenchymalandthelog-ranktest.TheexpressionlevelsoftranscriptionfactorTwist1andepithelial-totransitionmesenchymalTumortransition(EMT)makers(E-cad

6、herinandVimentin)weredetectedbyquantitativerealinvasiontimePCRandwesternblottinganalysisafterknockdownoflncRNAAFAP1-AS1.Results:TheexpressionlevelsoflncRNAAFAP1-AS1weresignificantlyelevatedinGBCtissuesandGBCcelllines.Inaddition,theexpressionleveloflncRNAAFAP1-AS1wassignificantlyassociatedw

7、ithtumorsizesandthehigherexpressionoflncRNAAFAP1-AS1wascorrelatedwithpoorprognosisinGBCpatients.KnockdownofLncRNAAFAP1-AS1suppressedcellgrowthandinvasioninNOZandGBC-SDcells.Furthermore,wefoundthatknockdownofLncRNAAFAP1-AS1inGBCcellsinhibitedEMTbydown-regulatingthetranscri