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1、Z-DeoxyglucosetransportandmetabolisminCaco-2cellsPIERREBISSONNETTE,HELENEGAGNE,ANNEBLAIS,ANDALFREDBERTELOOTMembranenansportResearchGroup,DepartmentofPhysiology,FacultyofMedicine,UniversityofMontreal,Montreal,QuebecH3C3J7,CanadaBissonnette,Pierre,H&meGag&,AnneBlais,andingthetranscellular
2、routeofglucoseabsorption(2).AlfredBerteloot.2DeoxyglucosetransportandmetabolismInterestingly,inthatrespect,ithasbeenshownthatinCaco-2cells.Am.J.Physiol.270(Gastrointest.LiverminimalmetabolismofluminalglucoseoccursduringPhysiol.33):Gl53-G162,1996.-Weinvestigatedthekinet-thetranscellularp
3、haseofactiveglucosetransport(28).icsof2deoxy-D-glucose(DG)uptakeandmetabolisminCaco-2cells,becausethishumancelllinemayrepresentaConsequently,theelaborationofarealisticcellularvalidenterocytemodeltoassessthedynamicsbetweensugarmodelofintestinalsugarabsorptionmayrequireconsid-transportand
4、metabolismandhencetoobtaininsightsintoerationofthedynamicsbetweenfreeintracellularthefactorsinvolvedduringtheintracellularphaseofglucoseglucoseandphosphorylatedsugarconcentrations.Suchabsorption.Whenstudiedin14-day-oldmonolayers,DGdynamicsarebeststudiedinaninvitrosystem.uptakeischaracte
5、rizedbyalagphasewithatimecourseThedemonstrationthatthehumancolonadenocarci-matchingthedecreaseinintracellularglucoseconcentra-tions,andnointracellularglucose6-phosphate(G-6-P)canbenomacelllineCaco-2issusceptibletospontaneousdetectedatanytimeduringincubation.After1hofpreincuba-enterocyti
6、cdifferentiation(23)recentlyresultedintionofCaco-2cellsinsubstrate-freetransportmedium,designationofthiscelllineasthemostrelevantinvitrohowever,steady-stateDGuptakematches2deoxy-D-glucosemodelforinvestigationsofdifferentiationandregula-6-phosphate(DG-6-P)accumulationwithundetectableleve
7、lstionofintestinalabsorbodigestivefunctions(26).UsingoffreeDG.ThiscomplexbehaviorinDGuptakeislinkedtoradiolabeledcx-methylglucose(a-MG)assubstrate(3),highhexokinaseactivityinCaco-2cells,andtheenzymehasweweresuccessfulinshowing,forthefirsttime,thataMichaelis-Mentenconstant(K,>fo
