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1、FoodandChemicalToxicology84(2015)29e36ContentslistsavailableatScienceDirectFoodandChemicalToxicologyjournalhomepage:www.elsevier.com/locate/foodchemtox7keto-stigmasteroland7keto-cholesterolinducedifferentialproteomechangestointestinalepitelial(Caco-2)cellsa,*bbbbJ.M
2、.Laparra,A.Alfonso-García,A.Alegría,R.Barbera,A.CillaaInstituteofFoodEngineeringforDevelopment,PolitechnicalUniversityofValencia,Avda.CamideVeras/n,46022Valencia,SpainbNutritionandFoodChemistry,FacultyofPharmacy,UniversityofValencia,Avda.VicenteAndresEstelless/n,
3、Burjassot,46100Valencia,SpainarticleinfoabstractArticlehistory:Recentstudieshaveexpandedtheappreciationoftherolesofoxysterolstriggeringinflammatory,im-Received15May2015munecytotoxicandapoptoticprocesses,buthavenotbeenconsideredforproteomeanalysis.AReceivedinrevisedfo
4、rmcomparativeproteomicstudyinintestinalepithelialcellculturesincubated(60mM/24h)with7keto-22June2015cholesterolor7keto-stigmasterolwasperformed.TheinfluenceofbothcompoundswasstudiedAccepted25June2015followingthenLC-TripleTOFanalysis.Findingswerecomparedtoresultsforco
5、ntrolcultures.IntheAvailableonline30June2015principalcomponentanalysis(PCA)ofproteomepatterns,twocomponentswereextractedaccountingfor99.8%ofthevarianceintheproteinexpression.PCAanalysisclearlydiscriminatedbetweentheKeywords:perturbationsintheproteomeofcellculturesin
6、cubatedwith7keto-cholesteroland7keto-stigmasterol.7keto-cholesterol7keto-stigmasterolTheseproteinsparticipateinmitochondrialfunction,lipidhomeostasis,inflammationandimmunityandOxysterolscellproliferation.Remarkabledifferencesbetweenproteomepatternsincellculturesexpos
7、edto7keto-Caco-2cellscholesteroland7keto-stigmasterolaffectmacrophagemigrationinhibitoryfactor,apolipoproteinE,Bcl-Proteome2-associatedtranscriptionfactorandcellularretinoicacid-bindingprotein.Besides,exposureto7keto-stigmasterolincreasedtheconcentrationofubiquitin-
8、conjugatingenzymeE2andthemitochondrialsuperoxidedismutaseprotein.Suchfindingsraisenewquestionsaboutsafetystudiesandtheregulatorypotentialof
