Reduction of Go phase cells of colon cancer caco-2 cells may enhance 5-fluorouracil efficacy

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1、Availableonlineatwww．sciencedirect．com8c⋯cE@。⋯cT。JBRJournalofBiomedicalResearch，2010，24(1)：64—68www．jbr-pub．orgResearchPaper覆一ReductionofG0phasecellsofcoloncancercaco一2cellsmayenhance5一fluorouraciIefficacy☆LinYe，KaixiongTao，YangYu，GuobinWangDepartmentofLapro

2、scopeswgeT，UnionHospitalaffiliatedtoHuaZhongUniversityofScienceandTechnology,WuHaa430022，ChinaRPivd1M~1ro~h201nAbstract0bjective：AmajorprobleminthechemotherapyofcoloncanermaybeduetothosecellsthatareinresidenceintheG0phasewheretheyarelessvulnerabletoconventio

3、naltherapy．Toovercomethisphenomenon，weattemptedtorecruitthereentry．ofthesecellsintothecellcycleviaasignalingpathwaythatmanipulatestumorgrowth．Methods：EpidermalgrowthfactorfEGF)wasusedtostimulatecoloncancercaco-2cells．FACSanalysisandproliferatingcellnuclearan

4、tigen(PCNA、stainingwereusedtoestimatethecellcycletransitionandcellproliferationactivatedbyEGF，andaMTTassaywasusedtoevaluatethesynergisticeflfectofEGFandchemotherapy．Results：Thepercentageofcaco-2cellsintheGo／G1phasewassignificantlyreducedbynearly20％andtheperc

5、entagesintheSandG。／MphaseswereincreasedbyEGF．ThecombineduseofEGFand5一fluorouracil(5一FU)enhancedthecaco一2cellchemosensitivityto5一FU．reachingamaximumofanapproximatelythreefoldgreatersensitivitythanto5一Fualoneasjudgedbythe50％inhibitingconcentration(IC5o)．Conclu

6、sion：OurstudydemonstratedthatstimulationbyEGFenhancedthechemosensitivityofcaco一2cellsto5一FU．whichmaybeanoveltherapeuticprotocolincoloncancer．Keywords：epidermalgrowthfactor，chemosensitivity，induction，dormantcellsINTR0DUCT10NcellsintheGnphase，cellsthatarelessv

7、ulnerabletocellcycledependentchemotherapyFurthermore，Coloncancerisstillaleadingcauseofcancer-thesecellsmaybeactivatedanytime，anywherebyrelatedmortalityintheworld．Althoughitsincidencegrowthfactorsorcytokines【7I8andmortalityhavedecreasedduetoremarkable．andthey

8、maybeatimebombfortumorrecurrence．Thisposesahurdleimprovementsinsurgicaltechnique，radiationtherapy，inthegOa1ofmoderncancertreatmentwherethegOalandsystemictherapieschemoresistanceisstillaistheprol