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1、AnalBioanalChem(2009)394:1117–1124DOI10.1007/s00216-008-2570-2REVIEWMicroRNAdetectionbymicroarrayWeiLi&KangchengRuanReceived:31October2008/Revised:1December2008/Accepted:5December2008/Publishedonline:9January2009#Springer-Verlag2009AbstractMicroRNAs(miRNAs)areaclassofsmallnon-targetmRNAs
2、[4–6].AsinglemicroRNAcanregulatethecodingRNAs∼22ntinlengththatregulategeneexpressionexpressionofmanytargetgenes,andatargetgenecanalsoandplayfundamentalrolesinmultiplebiologicalprocesses,beregulatedbyseveralmicroRNAs[7,8].Earlystudiesincludingcelldifferentiation,proliferationandapoptosisa
3、ssuggestedthatmicroRNAgenesaccountforabout1%ofwellasdiseaseprocesses.ThestudyofmiRNAhasthusthegenomeinaspecies[9,10].However,accordingtothebecomearapidlyemergingfieldinlifescience.Thestudyearlyin2005,thenumberofhumanmicroRNAsisdetectionofmiRNAexpressionisaveryimportantfirstgreaterthan100
4、0[11],andoverone-thirdofhumangenesstepinmiRNAexploration.Severalmethodologies,includ-appeartobeconservedmicroRNAtargets[12].ingcloning,northernblotting,real-timeRT-PCR,micro-Atpresent,microRNAsareknowntobeinvolvedinRNAarraysandISH(insituhybridization),havebeenmanybiologicalfunctions,incl
5、udingthetimingofearlydevelopedandappliedsuccessfullyinmiRNAprofiling.larvaldevelopmenttransitions[13,14],left/rightasymmetryThisreviewdiscussesthemainexistingmicroRNAdetec-ofchemoreceptorexpressioninnematodes[15],cellproli-tiontechnologies,whileemphasizingmicroRNAarrays.ferationandapopto
6、sisininsects[16],andhematopoieticdifferentiationinmammals[17],aswellasleafdevelopmentKeywordsMicroRNA.MicroRNAdetection.MicroRNA[18],floweringtransitiontiming[19],andflowerdevelop-microarray.MicroRNAlabelingmentofArabidopsis[20].Inaddition,microRNAsareassociatedwithmanymajordiseasesinhum
7、ans.Forexample,miR15andmiR16areassociatedwithhumanB-Introductioncellchroniclymphaticleukemia(B-CLL)[21],reducedexpressionoflet-7inlungcancerleadstoshortenedMicroRNAsareaclassof∼22ntlongnoncodingRNAs.postoperativesurvival[22],miR155isassociatedwithB-Theyarederivedfromthecl
