[英文原版 图形算法] microarray image analysis：an algorithmic approach：12. appendices

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1、12Appendices12.1AppendixA:MicroarrayvariantsAscanbeimagined,thereareseveralcompetingtechnologiesthatfacilitatethesimultaneousexaminationofthousandsofgenes.AppendixAhighlightssomeofthemaincontendersinthisrealm,alongwithrelevantmaterial.Thedigitalgenerationstageofthemicroarr

2、ayprocessisusuallycompletedwithaspeciallybuiltscannerdevice;thekeydifferencesbetweenthetwomostwidelyusedscannersystemsarehighlightedappropriately.12.1.1BuildingthechipsAlthoughthereareseveraldistinctstepsinthemicroarraychipbuildingsce-nario,thefirststeptypicallybeginswithcho

3、osinganappropriatesubstrate,whichhaspredictableandreproduciblecharacteristics.Toavoidamultitudeofprocessdifficultieswithregardstochemicalsandtoxicology,however,pre-treatedslideswithwellprovenprotocolmethodsarenormallypurchasedfromthirdpartiestoreducevariabilityproblems.Witht

4、hisbasematerialacquired,thenextstepistospotthegeneticmaterialofthetargetsontothesurfaceofthepre-printedprobesontheslide.Deoxyribonucleicacid(DNA)chipor“Chipfabrication”foramicroarraycantakeoneoftwodifferentapproaches.Eitherbeingprinteddirectlyontothebasematerial(asstatedint

5、hescenario)viaaroboticsystemoressentiallygrownviaphotolithographictechniques.Inthecaseofaglasssubstratebasematerial(themostcommonchoice),thesesystemscanalsobegroupedaccordingtotheattachmentchemistryusedtobondtheprobetotheslide.12.1.1.1RobotbasedRobotorcomplementarydeoxyrib

6、onucleicacid(cDNA)basedglassslide[185]systemsareconstructedinsuchawaythattheycontainthreedistinctareas.Thefirstconsistsoftheroboticarmandcontroller.Thisarmunitisnormallyastaticmatrixstructurewhichisattachedtotheoutershellofthemicroarraydevice.Inanidealsituation,duringanexpe

7、rimentrunthisarmistheonlycomponentthatwillmovewithinthemicroarray.However,thisisnotalwaysthecaseasthemicroarrayitselfisrarelyboltedtoastablecarrier.259260MicroarrayImageAnalysis:AnAlgorithmicApproachThesecondareaiscommonlycalledthehotel.Thisareacontainsthewellplates(384wel

8、lsaretypical)wherethemixedsamplessitinpreparationforthespottingprocess.Thereisalsoa“headc